Figure 5
From: Characterization of tamoxifen and 4-hydroxytamoxifen glucuronidation by human UGT1A4 variants
Western blot analysis of UGT1A4 protein from UGT1A4-overexpressing cell lines. Equal amounts (20 μg) of microsomal protein were loaded for each lane. The amount of UGT1A4 expression was normalized against the levels of calnexin in each lane and was quantified by densitometry relative as described in Materials and methods. Untransfected HK293 cell protein was analyzed as a negative control for UDP-glucuronosyltransferase detection.