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Figure 3 | Breast Cancer Research

Figure 3

From: The mTOR inhibitor rapamycin down-regulates the expression of the ubiquitin ligase subunit Skp2 in breast cancer cells

Figure 3

The effect of rapamycin on Skp2 protein levels and cell cycle distribution. (a) The effect of rapamycin on Skp2 protein levels. The breast cancer cell lines T47D and MDA-MB-231 were treated with rapamycin (20 nM) for different time periods and the expression of the protein was determined by western blot analysis. Skp1 was used as a loading control. (b) The time-dependent effect of rapamycin on G1 distribution in T47D and MDA-MB-231 cells. Cells were treated with rapamycin (20 nM) for different time periods and the percentage of cells in G1 was determined by FACS analysis using Modfit LT 2.0 software.

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