Figure 2

TGF-β₁ mediated EMT induces β₃ integrin:TβR-II complex formation on the cell surface of NMuMG cells. NMuMG cells were incubated in the absence or presence of TGF-β₁ (5 ng/ml) for 0–30 hours to induce EMT. Detergent-solubilized whole cell extracts (1 mg/tube) were immunoprecipitated with (a,b) anti-β₃ or (b) anti-β₁ integrin antibodies, and subsequently immunoblotted with anti-TβR-II antibodies. Shown are representative immunoblots from a single experiment that was repeated twice with identical results. EMT, epithelial-mesenchymal transitions; TβR; TGF receptor; TGF, transforming growth factor; WCE, whole cell extracts.