Figure 3
From: MUC1 alters oncogenic events and transcription in human breast cancer cells
Western blot analysis confirms changes in protein levels after MUC1 small interfering RNA (siRNA). (a) Whole cell lysates were analyzed for the following proteins: MUC1 extracellular subunit (MUC1-EX), c-Myc, total and active MAPK and ERK kinase (MEK)1/2 (MEK1/2, pMEK1/2), total and active extracellular signal regulated kinase (ERK)1/2 (ERK1/2, dpERK1/2), total and active AKT (AKT, pAKT), and tubulin as a loading control. Densitometry is shown for each blot, based on three independent measurements per band, normalized to tubulin; results are shown relative to luciferase siRNA (siLuc) for each cell line. Optimal exposures for comparing luciferase siRNA and MUC1 siRNA-treated cells from the same parental line are shown. (b) Lysates from epidermal growth factor (EGF)-stimulated MDA-MB-468 cells were blotted for MEK1/2, pMEK1/2, MUC1-EX, and actin (for loading).