Figure 1
From: MUC1 alters oncogenic events and transcription in human breast cancer cells
Small interfering RNA (siRNA) decreases MUC1 levels in breast cancer cells. (a) Western blots (IB) of the MUC1 cytoplasmic tail (MUC1-CT) and extracellular domain (MUC1-EX) in untreated (parental), or luciferase siRNA treated (siLuc) or MUC1 siRNA (siMUC1) treated MDA-MB-468 and BT-20 cells. Lysates were analyzed 48 hours post-transfection; tubulin is shown as a loading control. (b) Flow cytometry analysis of the MUC1 extracellular domain in unpermeabilized, siRNA-treated MDA-MB-468 and BT-20 lines. Cells were analyzed at 24 hour intervals beginning 48 hours post-transfection. The black line represents isotype control, the green luciferase siRNA (siLuc), and the red MUC1 siRNA (siMUC1).