Figure 1

IFN-γ secreting T cells in patients immunized with M-FP. Patient numbers 14, 5, 3 and 11 are shown. Responses to VNTR (pVNTR (which contains five VNTR repeats with no GST); black), PPD (internal positive control; grey) and no antigen (negative control; white). 1 = first injection; 2 = week 6 (2 weeks after the third injection); 3 = week 12 (2 weeks after the sixth injection); 4 = 6 months (3 months after the seventh injection); 5 = 9 months (3 months after the eighth injection); 6 = 1 year (3 months after the final (ninth) injection); 7 = 2 years, and 1 year and 3 months after the final injection; 8 = 3 years, and 2 years and 3 months after the final injection. GST, glutathione-S-transferase; IFN, interferon; M-FP, oxidized mannan conjugated to MUC1 fusion protein; PPD, purified protein derivative; SFU, spot forming units; VNTR, variable number of tandem repeats (from MUC1 sequence).