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Figure 3 | Breast Cancer Research

Figure 3

From: Type I insulin-like growth factor receptor over-expression induces proliferation and anti-apoptotic signaling in a three-dimensional culture model of breast epithelial cells

Figure 3

Increased proliferation and decreased luminal apoptosis in IGFIR structures. (a) Confocal sections of 34-day acinar structures from control (pCLXSN; panels a, c), and IGFIR-expressing (panels b, d) MCF-10A cells labeled with antibodies to the proliferative marker Ki-67 (green; panels a, b) or cleaved caspase-3 (green; panels c, d) and the nuclear counterstain TO-PRO-3 (blue). Scale bars = 50 μm. Arrows indicate a Ki-67-labeled cell (panel a) and a cleaved caspase-3 labeled cell (panel d). (b) Bar graph showing the percent of acini with three or more Ki-67-positive cells in control and IGFIR-expressing structures. Data are mean ± standard deviation from three different experiments grown for 20 days from two independently derived cell lines. At least 100 acini were counted in each experiment; the asterisk indicates significance, p < 0.01, Student's t test.

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Breast Cancer Research

ISSN: 1465-542X

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