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Table 1 PCR primer and SNP-specific fluorogenic oligonucleotide probe sequences

From: Germline polymorphisms in SIPA1are associated with metastasis and other indicators of poor prognosis in breast cancer

SIPA1 polymorphism Sequencea
-313G>A (rs931127) Forward primer 5'-GCGCGCTAGTGGAATGGTT-3'
  Reverse primer 5'-GACCAACCGGGAGGGCTA-3'
  Wild-type probe VIC-AGGGCCCAGTGCT
  Variant probe FAM-AGGGCCCAGTACTTA
545C>T (F182S; rs3741378) Reverse primer 5'-CCAGCTCGGACCTGCTGCAT-3'
  Reverse primer 5'-GATGGACACGGCCGCGTT-3'
  Wild-type probe VIC-ACCAGCATCCCCACC
  Variant probe FAM-ACCAGCATTCCCAC
2760G>A (A920A; rs746429) Forward primer 5'-CCTTGTGCCCACAGTCATGTC-3'
  Reverse primer 5'-CGGGACAGCGACTCCAGAATG-3'
  Wild-type probe VIC-CACTGCCCGCCTC
  Variant probe FAM-CCCACTGCCTGCC
  1. aFAM, 5-(&6)-carboxyfluorescein; SNP, single nucleotide polymorphism; VIC, Applied Biosystems® proprietary dye.
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Breast Cancer Research

ISSN: 1465-542X

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