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Table 1 PCR primer and SNP-specific fluorogenic oligonucleotide probe sequences

From: Germline polymorphisms in SIPA1are associated with metastasis and other indicators of poor prognosis in breast cancer

SIPA1 polymorphism

Sequencea

-313G>A (rs931127)

Forward primer

5'-GCGCGCTAGTGGAATGGTT-3'

 

Reverse primer

5'-GACCAACCGGGAGGGCTA-3'

 

Wild-type probe

VIC-AGGGCCCAGTGCT

 

Variant probe

FAM-AGGGCCCAGTACTTA

545C>T (F182S; rs3741378)

Reverse primer

5'-CCAGCTCGGACCTGCTGCAT-3'

 

Reverse primer

5'-GATGGACACGGCCGCGTT-3'

 

Wild-type probe

VIC-ACCAGCATCCCCACC

 

Variant probe

FAM-ACCAGCATTCCCAC

2760G>A (A920A; rs746429)

Forward primer

5'-CCTTGTGCCCACAGTCATGTC-3'

 

Reverse primer

5'-CGGGACAGCGACTCCAGAATG-3'

 

Wild-type probe

VIC-CACTGCCCGCCTC

 

Variant probe

FAM-CCCACTGCCTGCC

  1. aFAM, 5-(&6)-carboxyfluorescein; SNP, single nucleotide polymorphism; VIC, Applied Biosystems® proprietary dye.
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Breast Cancer Research

ISSN: 1465-542X

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