Figure 1

Differential CD24 expression distinguishes between mammary gland subtypes. (a) Cells were sorted on the basis of forward scatter and side scatter. (b) Dead cells (DAPI bright) were excluded. (c) B lymphocytes and immature T lymphocytes are CD24⁺ and, therefore, CD45 staining was used to exclude all leukocytes. (d) Doublets and higher order cell clumps were excluded using a time-of-flight approach (boxed cells represent single cells). (e) Typical CD24 staining profile, indicating the three populations of cells: CD24^High (69.9 ± 5.5%; n = 15), CD24^Low (22.2 ± 5.6%; n = 15) and CD24^Negative (6.3 ± 1.5%; n = 15). DAPI = 4',6-diamidino-2-phenylindole dihydrochloride; FITC, fluorescein isothiocyanate; PE-Cy5, phycoerythrin-Cy5.