Effect of tumor-derived supernatant (TDSN) and stimulation on expression of Bcl-2. (a)Expression of Bcl-2 in blood dendritic cells (DCs) was determined: ex vivo (Fresh); following culture (24 h) in the presence of 50% (v/v) peripheral blood mononuclear cell-conditioned supernatant (PBMC-SN) or TDSN (MA11); or following stimulation (24 h) with a cytokine cocktail (CC), synthetic double-stranded RNA (poly I:C), IL-12 or CD40 ligand (CD40L) prior (24 h) to culture with 50% (v/v) TDSN. Representative histograms (filled) are shown with numbers indicating the mean fluorescence intensity (MFI) of Bcl-2 expression. The unfilled histogram shows staining with isotype control. (b)Summary of Bcl-2 expression data. Results show the average ± standard error of the mean of five independent experiments for which statistically significant differences compared to fresh cells (*, p < 0.05; ***, p < 0.001) or after incubation with TDSN (##, p < 0.01; ###, p < 0.001) are indicated.