Figure 5

Effects of anti-estrogens and R115,777 on isoprenylated proteins in MCF-7 cells. Cells were incubated for 2 days with R115,777 (1 nM) combined with either tamoxifen (5 μM), ICI182777 (5 nM), N-pyrrolidine(-phenylmethyl-phenoxy)-ethanamine-HCl (PBPE; 10 μM) or estradiol (1 nM). Floating and adherent cell lysates were resolved by SDS-PAGE and blotted onto PVDF membranes and 5 μg protein were loaded for HDJ 2 detection, as described in Materials and methods. U, unprenylated; P, prenylated.