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Table 4 Array-based comparative genomic hybridization validation by DNA quantitative PCR of ERBB2

From: High resolution genomic analysis of sporadic breast cancer using array-based comparative genomic hybridization

Tumor ID DNA RNA Protein
  aCGH RP11-552K3 Light cycler TaqMan Hercept
  Cy3 Cy5 Relative ratio Relative alleles Ratio Staining
40 1.05 1.13 0.67 1 0.51 -
71 1.28 0.58 0.94 2 2.01 -
88 1.48 0.45 1.08 2 2.08 -
268 1.51 0.76 0.94 2 1.78 -
313 0.99 1 0.82 2 0.95 -
348 1.06 0.8 0.88 2 0.99 -
352 1.64 0.76 0.88 2 n.d.* -
476 1.03 0.88 0.94 2 1.72 -
493 1.25 0.97 0.88 2 n.d. -
277 1.14 0.99 0.94 2 0.67 2+
445 1.42 0.87 0.88 2 0.43 2+
316 2.41 0.43 1.51 3 5.61 3+
369 2.45 0.29 3.88 8 16.1 3+
  1. ERBB2 validation of array-based comparative genomic hybridization (aCGH) DNA copy number using LightCycler Her2/Neu DNA quantification kit (Roche), TaqMan Assay on Demand Hs. 00170433_m1 (Applied Biosystems), and protein quantification (Herceptest®). * n.d.: Not done