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Table 4 Array-based comparative genomic hybridization validation by DNA quantitative PCR of ERBB2

From: High resolution genomic analysis of sporadic breast cancer using array-based comparative genomic hybridization

Tumor ID

DNA

RNA

Protein

 

aCGH RP11-552K3

Light cycler

TaqMan

Hercept

 

Cy3

Cy5

Relative ratio

Relative alleles

Ratio

Staining

40

1.05

1.13

0.67

1

0.51

-

71

1.28

0.58

0.94

2

2.01

-

88

1.48

0.45

1.08

2

2.08

-

268

1.51

0.76

0.94

2

1.78

-

313

0.99

1

0.82

2

0.95

-

348

1.06

0.8

0.88

2

0.99

-

352

1.64

0.76

0.88

2

n.d.*

-

476

1.03

0.88

0.94

2

1.72

-

493

1.25

0.97

0.88

2

n.d.

-

277

1.14

0.99

0.94

2

0.67

2+

445

1.42

0.87

0.88

2

0.43

2+

316

2.41

0.43

1.51

3

5.61

3+

369

2.45

0.29

3.88

8

16.1

3+

  1. ERBB2 validation of array-based comparative genomic hybridization (aCGH) DNA copy number using LightCycler Her2/Neu DNA quantification kit (Roche), TaqMan Assay on Demand Hs. 00170433_m1 (Applied Biosystems), and protein quantification (Herceptest®). * n.d.: Not done
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Breast Cancer Research

ISSN: 1465-542X

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