Comparison of gene expression profiles in breast cancer patient samples and normal human fibroblasts. Messenger RNA was extracted from (a) human primary breast cancer samples from eight patients and (b) human primary fibroblasts from three patients and reverse-transcribed into cDNA. Real-time PCR was performed to evaluate the expression of the genes encoding cyclooxygenase (Cox)-2, epithelial glycoprotein (EGP)-2, secretory leukoprotease inhibitor (SLPI), survivin and the α-chemokine SDF-1 receptor (CXCR4). The mRNA copy numbers are normalized by the glyceraldehyde-3-phosphate dehydrogenase copy number. Each bar presents the mean ± standard deviation. Asterisks indicate p < 0.05 for breast cancer versus normal fibroblasts.