Figure 4

CCN6 inhibition increases the growth of human mammary epithelial cells in response to insulin-like growth factor-1 (IGF-1). Cells were serum starved for 24 hours, and subsequently stimulated with 25 ng/ml of IGF-1. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) reagents were added 24 hours later and the plate was read at a wavelength of 595 nm. Results are expressed as means ± SD for three independent experiments. Asterisks indicate statistical significance (t test); p < 0.005 for short interfering RNA (siRNA)/CCN6 after treatment with IGF-1 compared with serum free medium (SFM). C1 and C2 are two stable CCN6 deficient clones.