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Table 1 Oligonucleotides used in PCR amplification

From: Effects of combined treatment with rapamycin and cotylenin A, a novel differentiation-inducing agent, on human breast carcinoma MCF-7 cells and xenografts

  Sense strand Antisense strand
TGFBI 5'-TGTGTGCTGTGCAGAAGGTT-3' 5'-ATATGGTAGCGGAGGGCATT-3'
BIK 5'-CTCCAGAGACATCTTGATGG-3' 5'-TGGGATCTCCAGAACCTCATT-3'
Cyclin G2 5'-AGCACTTGGCAGGTCATGAA-3' 5'-CAACTATTCTAGCAGCCAGC-3'
GRB7 5'-TCTGCCTGAGGAGGTAAAGA-3' 5'-GGAGCTCTTGAACAGTTCGT-3'
EGR3 5'-CTACTTGGGAAAGTTCGCCT-3' 5'-GAATGCCTTGATGGTCTCCA-3'
GAPDH 5'-GGTCGGAGTCAACGGATTTG-3' 5'-ATGAGCCCCAGCCTTCTCCAT-3'
  1. BIK, BCL2-interacting killer; EGR, early growth response; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GRB, growth factor receptor-bound; TGFBI, transforming growth factor-β-induced 68 kDa