Figure 7
Time courses of TGFBI, BIK, cyclin G2 and GRB7 mRNA expression in MCF-7 cells. (a) MCF-7 cells were treated without (lanes 1, 5, 9, 13 and 17) or with 0.5 ng/ml rapamycin (lanes 2, 6, 10, 14 and 18), 10 μg/ml CN-A (lanes 3, 7, 11, 15 and 19), or 0.5 ng/ml rapamycin plus 10 μg/ml CN-A (lanes 4, 8, 12, 16 and 20) for 1, 3, 6, 12 and 24 hours. Expression of mRNAs was examined by RT-PCR analysis. (b) Graphs depict the relative levels of mRNA after normalization to GAPDH mRNA levels in Fig. 5a. Cells were treated with 0.5 ng/ml rapamycin (white triangle) or 10 μg/ml CN-A (white square), or both combined (black circle). BIK, BCL2-interacting killer; CN-A, cotylenin A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GRB, growth factor receptor-bound; TGFBI, transforming growth factor-β-induced 68 kDa protein.