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Figure 6 | Breast Cancer Research

Figure 6

From: Effects of combined treatment with rapamycin and cotylenin A, a novel differentiation-inducing agent, on human breast carcinoma MCF-7 cells and xenografts

Figure 6

Effects of rapamycin and CN-A on the expressions of p53, p21Cip1, p27Kip1 and cyclins. Expression of mRNAs was examined by RT-PCR analysis. (a) MCF-7 cells were cultured with 2.5–10 μg/ml CN-A or 1 ng/ml rapamycin, or both combined for 24 hours. (b) MCF-7 cells were cultured with 0.5 ng/ml rapamycin or 10 μg/ml CN-A, or both combined for 12 hours. The levels of GAPDH expression are shown to demonstrate that equal amounts of RNA were used for RT-PCR. CN-A, cotylenin A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

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