From: Mutation analysis of FANCD2, BRIP1/BACH1, LMO4 and SFN in familial breast cancer
Gene | Exon | Forward primer | Reverse primer | Annealing temp (°C) | Amplicon size (bp) | DHPLC temp (°C) |
---|---|---|---|---|---|---|
FANCD2 | 1 | GGTGAGCCCAGCTTATTGC | GCCTCGAGAGACTACGACCA | 55 | 188 | 64 |
 | 2 | CTTCAGCAACAGCGAAGTAGTCTG | GATTCTCAGCACTTGAAAAGCAGG | 50 | 421 | 56 |
 | 3 | GGACACATCAGTTTTCCTCTC | GAAAACCCATGATTCAGTCC | 50 | 308 | 56 |
 | 4 & 5 | TCATCAGGCAAGAAACTTGG | GAAGTTGGCAAAACAGACTG | 50 | 466 | 53, 58 |
 | 6 | GAGCCATCTGCTCATTTCTG | CCCGCTATTTAGACTTGAGC | 50 | 282 | 56 |
 | 7 | CAAAGTGTTTATTCCAGGAGC | CATCAGGGTACTTTGAACATTC | 50 | 342 | 55, 60 |
 | 8 | GACCAGAAAGGCTCAGTTCC | CTGCAGCACATTACCCAGAA | 55 | 463 | 51, 56 |
 | 9 | CAGCTCTGCATTTCACACGTA | TGCAGTGATAGAACCCCCATA | 55 | 265 | 60, 62 |
 | 10 | TGCCCAGCTCTGTTCAAACC | AGGCAATGACTGACTGACAC | 50 | 219 | 59 |
 | 11 | TGCCCGTCTATTTTTGATGAAGC | TCTCAGTTAGTCTGGGGACAG | 50 | 391 | 56 |
 | 12 | TCATGGTAGAGAGACTGGACTGTGC | ACCCTGGAGCAAATGACAACC | 55 | 432 | 53, 58 |
 | 13 | ATGGCAGGAACTCCGATCTT | CCTTATTCAGCATGCCCTGT | 55 | 363 | 56 |
 | 14 | GGGCATGCTGAATAAGGTGT | GACAGTGGGAAGGCAAGCTA | 55 | 189 | 57 |
 | 15 | GGGAGTGTGTGGAACAAATGAGC | AGTTTCTACAGGCTGGTCCTATTCC | 50 | 513 | 56 |
 | 16 | AACGTGGAATCCCATTGATGC | TTTCTGTGTTCCCTCCTTGC | 58 | 378 | 57 |
 | 17 | GATGGTCAAGTTACACTGGC | CACCTCCCACCAATTATAGTATTC | 54 | 381 | 57 |
 | 18 | CTATGTGTGTCTCTTTTACAGGG | AATCTTTCCCACCATATTGC | 50 | 233 | 52, 57 |
 | 19 | CATACCTTCTTTTGCTGTGC | CCACAGAAGTCAGAATCTCCACG | 48 | 198 | 59 |
 | 20 | ACACACCGGGGAATGTTGT | CTGCAGGTTGGGTGACAGAG | 53 | 341 | 49, 54, 59 |
 | 21 | GAGTTTGGGAAAGATTGGCAGC | TGTAGTAAAGCAGCTCTCATGC | 54 | 231 | 59 |
 | 22 | ACACTCTGCACTGCCCTTTT | GAAAGAAGCTGTGGGACTGG | 55 | 239 | 60 |
 | 23 | TGGTTTTCCCTGTAGCCTTG | CTCAACTTCCCCACCAAGAG | 55 | 294 | 60 |
 | 24 | TTGTGTTCCCTATGTATGTGGAG | TTGATCTGCCCTTTCCCTAA | 54 | 269 | 58 |
 | 25 | CAGCTAGCTCCAGAGGCAAC | TGCACAATAAGTGAAACATCCT | 54 | 229 | 58 |
 | 26 | TTCATAGACATCTCTCAGCTCTG | GTTTTGGTATCAGGGAAAGC | 52 | 283 | 52, 57 |
 | 27 | GCCATGCTTGGTAATTTTGG | TTCCAATTACTGATGCCATGAT | 55 | 319 | 57 |
 | 28 | TTGTTTTCTGAGGGCAATGA | TGGGATGTCACAAACCTTCA | 55 | 230 | 52, 57 |
 | 29 | GGTCTTGATGTGTGACTTGTATCCC | CCTCAGTGTCACAGTGTTCTTTGTG | 58 | 446 | 51, 56 |
 | 30 | CATGAAATGACTAGGACATTCC | CTACCCAGTGACCCAAACAC | 52 | 280 | 60 |
 | 31 | GACTCCATTGCGAACCCTTA | CAGCCAAAAATGAAACCTCA | 55 | 260 | 58 |
 | 32 | TGAGGTTTCATTTTTGGCTGA | GTGCCTTGGTGACTGTCAAA | 55 | 268 | 58 |
 | 33 | TTGATGGTACAGACTGGAGGC | AAGAAAGTTGCCAATCCTGTTCC | 50 | 273 | 58 |
 | 34 | AGCACCTGAAAATAAGGAGG | GCCCAAAGTTTGTAAGTGTGAG | 52 | 342 | 52, 57 |
 | 35 | TGTTAGACCGGGAACGTCTT | TTGGCTCATTGGAAGAAACC | 55 | 257 | 54, 59 |
 | 36 | TCTTCCAATGAGCCAAATAGC | CCCAGCCTGATGATGATTCT | 55 | 450 | 58 |
 | 37 | AGAGGTAGGGAAGGAAGCTAC | CCAAAGTCCACTTCTTGAAG | 50 | 232 | 60 |
 | 38 | GATGCACTGGTTGCTACATC | CCAGGACACTTGGTTTCTGC | 52 | 274 | 58 |
 | 39 | ACACTCCCAGTTGGAATCAG | CTTGTGGGCAAGAAATTGAG | 54 | 369 | 54, 59 |
 | 40 | TGGGCTGGATGAGACTATTC | CCAAGGACATATCTTCTGAGCAAC | 52 | 222 | 59 |
 | 41 | TGATTATCAGCATAGGCTGG | GATCCCCCAATAGCAACTGC | 52 | 270 | 54, 59 |
 | 42 | CATTCAGATTCACCAGGACAC | CCTTACATGCCATCTGATGC | 54 | 226 | 59 |
 | 43 | AACCTTCTCCCCTATTACCC | GGAAAATGAGAGGCTATAATGC | 52 | 434 | 58 |
 | 44 | TGTATTCCAGAGGTCACCCAGAGC | CCAGTAAGAAAGGCAAACAGCG | 59 | 234 | 58 |
BRIP1/ BACH1 | 2 | TTCTTTGTAAGGCGTGTCTCAA | ATACTCAATGTACTTTATGGGTCA | 50 | 246 | 55 |
 | 3 | TTGTTGTGTTCCCTGGAGTG | GACAGCATGGCTGAACCAGT | 55 | 325 | 56 |
 | 4 | CTGGGTGAACTGGGCTGTAG | CAACTGACCCAGGCAAAATA | 55 | 281 | 52, 57 |
 | 5 | AAGCCTTACATCCTGGTTCC | CTAACTGGGTTATTTACTGCCAAT | 54 | 254 | 55 |
 | 6 | GAGCTGTTTTGGCCTTTGAG | TTCCTTCTTTAAAACTGAACAATGG | 55 | 239 | 56 |
 | 7 | TTCCATGTGAGGTTTGATAACG | GCAGTTAATTTGATTTTCCGAAG | 55 | 481 | 53, 58 |
 | 8 | GCCTTGTGGCTTTAATGATG | CATCTCCATGAGTAGGAAGAAGGT | 55 | 373 | 57 |
 | 9 | TGAAATATCTTGCCTGCTGTTG | TTTTTCATATAAAGGCAGCACAA | 55 | 321 | 52, 57 |
 | 10 | GATCAACGCATGACAATAATGA | AATTCACTAAATACGTTTCACAGGT | 50 | 270 | 55 |
 | 11 | CCCTCCCAACCCCTCTATAC | TGCTAGCATCCAAATTAGGCTAT | 55 | 258 | 54 |
 | 12 | TGGGAAGGTACCAGCTCTTT | TGCTGGTACTGAGCAAGAAGAC | 55 | 333 | 56 |
 | 13 | TCAGACTCCTAGACTCAAGCGAT | TTACTTGCTGGCACTTCAGG | 54 | 364 | 54, 59 |
 | 14 | CATGCCTTTTTCAGGCATAA | TTGCCTCTACCCTAGGAAGC | 50 | 318 | 53, 58 |
 | 15 | GCCGTAGTCACATTGGCTTA | TTTTCACCAGACCATGAAATAA | 50 | 278 | 56 |
 | 16 | TTCTGAGAATTTTGGATATGCTTTT | TGCTGAAAGACTTGCACAATG | 55 | 319 | 57 |
 | 17 | TTGAGATCACACAGCTGATTAGTTA | CCAGTTCCTATGGTTCCAGTT | 55 | 458 | 50, 55 |
 | 18 | CTGTCCCACTGGAAAACTGG | TCTGCTGTGAAATACTGTGCTT | 50 | 297 | 54 |
 | 19 | GGTTACTTCACTAGAAAAAGCAAGTG | TCAAAGGTAAATGGGAAGAACTTT | 55 | 456 | 56 |
 | 20a | GCAATTATGTTAGCTAGGAGCAGA | TCAAAATCTCTATTTGAAGTGGACTG | 55 | 516 | 52, 57 |
 | 20b | TGAACATCCGCTCTGTTCTG | TGCAATCCTCAGCTTTCACT | 55 | 305 | 55 |
 | 20c | AACAATTCAGATTGCATTTTAGC | GAGTTTAACATAAGCATGATGAC | 50 | 328 | 53 |
LMO4 | 2 | TTTCTCTCCCTGTCCCCTTC | CCCCATCTCCAGGAAAGAC | 55 | 304 | 59,64 |
 | 3 | CCAAAGGGATGCCCAGAGT | GCCCCCATGTAGGTAGGAAA | 64 | 254 | 55,60 |
 | 4 | CCTTGTTTTCAGTGGGTTTG | GGTTGAACCTTCCAACTTGC | 55 | 246 | 59 |
SFN | 1a | GCCAAGAGCAGGAGAGACAC | ATGCGCTTCTTGTCGTCAC | 60 | 510 | 63 |
 | 1b.1 | GAGAGCCGGGTCTTCTACCT | TGATGAGGGTGCTGTCTTTG | 62 | 316 | 62 |
 | 1b.2 | TCTCTCTGGCCAAGACCACT | TCCCACCCCATACTAGTCCTC | 64 | 249 | 63 |