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Table 1 FANCD2, BRIP1/BACH1, LMO4 and SFN primers

From: Mutation analysis of FANCD2, BRIP1/BACH1, LMO4 and SFN in familial breast cancer

Gene

Exon

Forward primer

Reverse primer

Annealing temp (°C)

Amplicon size (bp)

DHPLC temp (°C)

FANCD2

1

GGTGAGCCCAGCTTATTGC

GCCTCGAGAGACTACGACCA

55

188

64

 

2

CTTCAGCAACAGCGAAGTAGTCTG

GATTCTCAGCACTTGAAAAGCAGG

50

421

56

 

3

GGACACATCAGTTTTCCTCTC

GAAAACCCATGATTCAGTCC

50

308

56

 

4 & 5

TCATCAGGCAAGAAACTTGG

GAAGTTGGCAAAACAGACTG

50

466

53, 58

 

6

GAGCCATCTGCTCATTTCTG

CCCGCTATTTAGACTTGAGC

50

282

56

 

7

CAAAGTGTTTATTCCAGGAGC

CATCAGGGTACTTTGAACATTC

50

342

55, 60

 

8

GACCAGAAAGGCTCAGTTCC

CTGCAGCACATTACCCAGAA

55

463

51, 56

 

9

CAGCTCTGCATTTCACACGTA

TGCAGTGATAGAACCCCCATA

55

265

60, 62

 

10

TGCCCAGCTCTGTTCAAACC

AGGCAATGACTGACTGACAC

50

219

59

 

11

TGCCCGTCTATTTTTGATGAAGC

TCTCAGTTAGTCTGGGGACAG

50

391

56

 

12

TCATGGTAGAGAGACTGGACTGTGC

ACCCTGGAGCAAATGACAACC

55

432

53, 58

 

13

ATGGCAGGAACTCCGATCTT

CCTTATTCAGCATGCCCTGT

55

363

56

 

14

GGGCATGCTGAATAAGGTGT

GACAGTGGGAAGGCAAGCTA

55

189

57

 

15

GGGAGTGTGTGGAACAAATGAGC

AGTTTCTACAGGCTGGTCCTATTCC

50

513

56

 

16

AACGTGGAATCCCATTGATGC

TTTCTGTGTTCCCTCCTTGC

58

378

57

 

17

GATGGTCAAGTTACACTGGC

CACCTCCCACCAATTATAGTATTC

54

381

57

 

18

CTATGTGTGTCTCTTTTACAGGG

AATCTTTCCCACCATATTGC

50

233

52, 57

 

19

CATACCTTCTTTTGCTGTGC

CCACAGAAGTCAGAATCTCCACG

48

198

59

 

20

ACACACCGGGGAATGTTGT

CTGCAGGTTGGGTGACAGAG

53

341

49, 54, 59

 

21

GAGTTTGGGAAAGATTGGCAGC

TGTAGTAAAGCAGCTCTCATGC

54

231

59

 

22

ACACTCTGCACTGCCCTTTT

GAAAGAAGCTGTGGGACTGG

55

239

60

 

23

TGGTTTTCCCTGTAGCCTTG

CTCAACTTCCCCACCAAGAG

55

294

60

 

24

TTGTGTTCCCTATGTATGTGGAG

TTGATCTGCCCTTTCCCTAA

54

269

58

 

25

CAGCTAGCTCCAGAGGCAAC

TGCACAATAAGTGAAACATCCT

54

229

58

 

26

TTCATAGACATCTCTCAGCTCTG

GTTTTGGTATCAGGGAAAGC

52

283

52, 57

 

27

GCCATGCTTGGTAATTTTGG

TTCCAATTACTGATGCCATGAT

55

319

57

 

28

TTGTTTTCTGAGGGCAATGA

TGGGATGTCACAAACCTTCA

55

230

52, 57

 

29

GGTCTTGATGTGTGACTTGTATCCC

CCTCAGTGTCACAGTGTTCTTTGTG

58

446

51, 56

 

30

CATGAAATGACTAGGACATTCC

CTACCCAGTGACCCAAACAC

52

280

60

 

31

GACTCCATTGCGAACCCTTA

CAGCCAAAAATGAAACCTCA

55

260

58

 

32

TGAGGTTTCATTTTTGGCTGA

GTGCCTTGGTGACTGTCAAA

55

268

58

 

33

TTGATGGTACAGACTGGAGGC

AAGAAAGTTGCCAATCCTGTTCC

50

273

58

 

34

AGCACCTGAAAATAAGGAGG

GCCCAAAGTTTGTAAGTGTGAG

52

342

52, 57

 

35

TGTTAGACCGGGAACGTCTT

TTGGCTCATTGGAAGAAACC

55

257

54, 59

 

36

TCTTCCAATGAGCCAAATAGC

CCCAGCCTGATGATGATTCT

55

450

58

 

37

AGAGGTAGGGAAGGAAGCTAC

CCAAAGTCCACTTCTTGAAG

50

232

60

 

38

GATGCACTGGTTGCTACATC

CCAGGACACTTGGTTTCTGC

52

274

58

 

39

ACACTCCCAGTTGGAATCAG

CTTGTGGGCAAGAAATTGAG

54

369

54, 59

 

40

TGGGCTGGATGAGACTATTC

CCAAGGACATATCTTCTGAGCAAC

52

222

59

 

41

TGATTATCAGCATAGGCTGG

GATCCCCCAATAGCAACTGC

52

270

54, 59

 

42

CATTCAGATTCACCAGGACAC

CCTTACATGCCATCTGATGC

54

226

59

 

43

AACCTTCTCCCCTATTACCC

GGAAAATGAGAGGCTATAATGC

52

434

58

 

44

TGTATTCCAGAGGTCACCCAGAGC

CCAGTAAGAAAGGCAAACAGCG

59

234

58

BRIP1/ BACH1

2

TTCTTTGTAAGGCGTGTCTCAA

ATACTCAATGTACTTTATGGGTCA

50

246

55

 

3

TTGTTGTGTTCCCTGGAGTG

GACAGCATGGCTGAACCAGT

55

325

56

 

4

CTGGGTGAACTGGGCTGTAG

CAACTGACCCAGGCAAAATA

55

281

52, 57

 

5

AAGCCTTACATCCTGGTTCC

CTAACTGGGTTATTTACTGCCAAT

54

254

55

 

6

GAGCTGTTTTGGCCTTTGAG

TTCCTTCTTTAAAACTGAACAATGG

55

239

56

 

7

TTCCATGTGAGGTTTGATAACG

GCAGTTAATTTGATTTTCCGAAG

55

481

53, 58

 

8

GCCTTGTGGCTTTAATGATG

CATCTCCATGAGTAGGAAGAAGGT

55

373

57

 

9

TGAAATATCTTGCCTGCTGTTG

TTTTTCATATAAAGGCAGCACAA

55

321

52, 57

 

10

GATCAACGCATGACAATAATGA

AATTCACTAAATACGTTTCACAGGT

50

270

55

 

11

CCCTCCCAACCCCTCTATAC

TGCTAGCATCCAAATTAGGCTAT

55

258

54

 

12

TGGGAAGGTACCAGCTCTTT

TGCTGGTACTGAGCAAGAAGAC

55

333

56

 

13

TCAGACTCCTAGACTCAAGCGAT

TTACTTGCTGGCACTTCAGG

54

364

54, 59

 

14

CATGCCTTTTTCAGGCATAA

TTGCCTCTACCCTAGGAAGC

50

318

53, 58

 

15

GCCGTAGTCACATTGGCTTA

TTTTCACCAGACCATGAAATAA

50

278

56

 

16

TTCTGAGAATTTTGGATATGCTTTT

TGCTGAAAGACTTGCACAATG

55

319

57

 

17

TTGAGATCACACAGCTGATTAGTTA

CCAGTTCCTATGGTTCCAGTT

55

458

50, 55

 

18

CTGTCCCACTGGAAAACTGG

TCTGCTGTGAAATACTGTGCTT

50

297

54

 

19

GGTTACTTCACTAGAAAAAGCAAGTG

TCAAAGGTAAATGGGAAGAACTTT

55

456

56

 

20a

GCAATTATGTTAGCTAGGAGCAGA

TCAAAATCTCTATTTGAAGTGGACTG

55

516

52, 57

 

20b

TGAACATCCGCTCTGTTCTG

TGCAATCCTCAGCTTTCACT

55

305

55

 

20c

AACAATTCAGATTGCATTTTAGC

GAGTTTAACATAAGCATGATGAC

50

328

53

LMO4

2

TTTCTCTCCCTGTCCCCTTC

CCCCATCTCCAGGAAAGAC

55

304

59,64

 

3

CCAAAGGGATGCCCAGAGT

GCCCCCATGTAGGTAGGAAA

64

254

55,60

 

4

CCTTGTTTTCAGTGGGTTTG

GGTTGAACCTTCCAACTTGC

55

246

59

SFN

1a

GCCAAGAGCAGGAGAGACAC

ATGCGCTTCTTGTCGTCAC

60

510

63

 

1b.1

GAGAGCCGGGTCTTCTACCT

TGATGAGGGTGCTGTCTTTG

62

316

62

 

1b.2

TCTCTCTGGCCAAGACCACT

TCCCACCCCATACTAGTCCTC

64

249

63

  1. DHPLC, denaturing high performance liquid chromatography.