Figure 5

Ectopic dominant active Rac(G12V) expression in low metastatic variant MDA-MB-435Br1. Confocal DIC (Differential Interference Contrast) and fluorescent microscopy was performed on MDA-MB-435Br1 cell variant stably expressing vector alone, myc-Rac1(G12V), or myc-Rac3(G12V). Cells were plated on glass coverslips, fixed in 3.7% formaldehyde and permeablized with 0.2% Triton X-100. Actin was then visualized with rhodamine phalloidin and focal adhesions were visualized with an anti-p-tyro antibody followed by an FITC conjugate.