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Figure 3 | Breast Cancer Research

Figure 3

From: Rac1 and Rac3 isoform activation is involved in the invasive and metastatic phenotype of human breast cancer cells

Figure 3

Rac and Cdc42 activity in MDA-MB-435 metastatic variants. Whole cell lysates of all variants were subjected to SDS-PAGE followed by western blot analysis for (a) total Rac using an anti-Rac antibody and (b) total Cdc42 using an anti-Cdc42 antibody. Rac and Cdc42 activities were assayed using the PAK-PBD activity assay. A non-hydrolyzable GTP analog, GTPγS, was used as the positive control; GDP alone was used for the negative control. Equal loading of lanes was maintained by performing a total protein assay and is confirmed by western blot analysis for total actin. Results are representative of three to five independent experiments.

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