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Figure 1 | Breast Cancer Research

Figure 1

From: Rac1 and Rac3 isoform activation is involved in the invasive and metastatic phenotype of human breast cancer cells

Figure 1

Characterization of cytoskeletal structures and focal adhesion distribution in MDA-MB-435 metastatic variants. (a) Each of the MDA-MB-435 metastatic variants was plated onto glass coverslips. Actin was visualized with rhodamine phalloidin and focal adhesions were visualized with an anti-p-tyro antibody followed by an FITC conjugated secondary antibody. (b) Cell area was measured on 50 individual cells per variant using Spot Digital Camera Software. Focal adhesion number was divided by cell area and plotted on the y-axis. Bars represent ±SEM, and are representative of three independent experiments. Treatments denoted by the same letter indicate no significant difference between those treatments. Treatments denoted by different letters indicate a significant difference between those treatments (P < 0.01).

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