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Figure 6 | Breast Cancer Research

Figure 6

From: Clioquinol and pyrrolidine dithiocarbamate complex with copper to form proteasome inhibitors and apoptosis inducers in human breast cancer cells

Figure 6

Clioquinol (CQ) + copper and pyrrolidine dithiocarbamate (PDTC) + copper fail to inhibit proteasome activity in normal MCF 10 (MCF-10A) cells. Normal MCF-10A and malignant mCF-10 (DCIS) breast cells were treated with 20 μM of copper (Cu), CQ, CQ + copper (CC), tetrathiomolybdate (TM), TM + copper (TC), or 5 μM PDTC or PDTC + copper (PC). Dimethylsulfoxide (D) was used as a control. After 24 h, cells were collected and lysed. Lysates were analyzed for chymotrypsin-like activity and showed as released fluorescence units (RFUs). The complexes were capable of eliminating proteasome activity in DCIS cells but not in MCF-10A cells. This strongly suggests that these metal complexes do not inhibit proteasome activity in normal breast cells.

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Breast Cancer Research

ISSN: 1465-542X

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