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Table 2 Comparative results of screening methods to detect the ER-α A908G mutation

From: The estrogen receptor-α A908G (K303R) mutation occurs at a low frequency in invasive breast tumors: results from a population-based study

CBCS tumor

33P-cycle sequencing

Fluorescent sequencing

SNaPshot primer extension

28

+

-

+

36

+

-

+b

313

+

-

+

536

+

-

+

622

+a

-

+

684

+

-

+

705

+

-

+b

710

+a

-

-

162

-

-

-

297

-

-

-

Positive control tissue

+

-

+

Mutant clone from positive control

+

+

n.d.

  1. aThe mutation was also confirmed by 35S manual sequencing in these samples; other tumors shown were not evaluated by this method.
  2. bMutant peaks were small, indicating low-level positivity.
  3. CBCS, Carolina Breast Cancer Study; ER, estrogen receptor; n.d., not determined.