Figure 6

In vitro α-difluoromethylornithine (DFMO) treatment decreases meprin α expression in MDA-MB-435 cells, but not in MDA-MB-231 cells. End-point RT-PCR analysis of total RNA from (a) MDA-MB-231 cells or (b) MDA-MB-435 cells, either left untreated (C), treated with 1 mM DFMO for 48 hours (D) or treated with 1 mM DFMO plus 2.5 mM putrescine for 48 hours (D + P). Quantities of meprin α mRNA in (c) MDA-MB-231 cells or (d) MDA-MB-435 cells were normalized to glyceraldehyde-3-phosphate dehydrogenase levels (GADPH) – normalization to hypoxanthine phosphoribosyl transferase [HPRT] was also performed in (a). Meprin α expression levels are graphed as a percentage of control.