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Figure 4 | Breast Cancer Research

Figure 4

From: Proteomic identification of heat shock protein 90 as a candidate target for p53 mutation reactivation by PRIMA-1 in breast cancer cells

Figure 4

Protein-protein interaction analysis of p53 and the α isoform of heat shock protein 90 (Hsp90α). (a) MDA-231 cell lysates from untreated cells (lanes 1 and 3) and cells treated for 4 hours with 100 μM PRIMA-1 (lanes 2 and 4) were immunoprecipitated (IP) with anti-Hsp90α monoclonal antibody and subjected to Western blotting (WB) with anti-p53 (DO-1) monoclonal antibody (lanes 1 and 2) in addition to reciprocal immunoprecipitation with DO-1 and Western blotting with anti-Hsp90α (lanes 3 and 4). (b) GI-101A cell lysates from untreated cells (lanes 1 and 3) and cells treated for 4 hours with 100 μM PRIMA-1 (lanes 2 and 4) were immunoprecipitated with anti-Hsp90α monoclonal antibody and subjected to Western blotting with anti-p53 (DO-1) monoclonal antibody (lanes 1 and 2) in addition to reciprocal immunoprecipitation with DO-1 and Western blotting with anti-Hsp90α (lanes 3 and 4).

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