Patient no. | FISHa | Western blotb | LRIG1 RT-PCR (T/NN)c |
|---|
B1 | I | In | 1.45d |
B2e | I | In | - |
B3e | NL | E | - |
B4 | NL | D | 0.85 |
B5 | I | In | 1.63d |
B6 | NL | E | 0.50d |
B7 | NL | In | 0.36d |
B8 | I | In | 1.13 |
B9 | NL | E | 1.17 |
- aFISH: I, increased LRIG1 copy number; NL, normal LRIG1 copy number. bWestern blot: comparison of the staining in tumour tissue (T) and non-neoplastic tissue (NN). A visual increase (In)/decrease (D) of at least 50% was considered convincing. E, equal levels of protein. cLRIG1 RT-PCR T/NN; LRIG1 mRNA expression level in neoplastic (T) tissue samples divided by levels in matched non-neoplastic (NN) tissue samples. dAs described in the results, at p.9, ratios >1.2 are regarded as significant overexpression and ratios <0. 8 are regarded as significant underexpression in neoplastic tissue compared to non-neoplastic tissue. Samples were consecutively collected. eTwo tumours did not yield RNA of sufficient quality for the RT-PCR analysis.
