Figure 2
From: The expression of the ubiquitin ligase subunit Cks1 in human breast cancer
Immunoblots showing Cks1, Skp2, and p27Kip1 proteins in breast cancer cell lines. (a) Levels of the three proteins Cks1 (cyclin kinase subunit 1), Skp2 (S-phase kinase protein 2), and p27Kip1 were measured in MDA-MB-231 (an estrogen-independent cell line) and MCF-7 (an estrogen-dependent cell line). Skp1 levels were determined to confirm equal protein loading. (b)Effect of estrogen modulation on Cks1, Skp2, and p27Kip1 expression in T47D cells (an estrogen-dependent breast cancer cell line) treated with estradiol (0.5 μM) or tamoxifen (10 μM). P27, p27Kip1.