Figure 3
From: Somatic mutation and gain of copy number of PIK3CA in human breast cancer
Gain of PIK3CA gene copy number in breast cancer. (a)The change in the gene copy number of PIK3CA in 33 paired breast tissue samples. *Cases with significant change in gene copy number. The case number is indicated below the panel. (b) The change in the gene copy number of PIK3CA in 92 breast tumors and 33 normal controls. (c) Eight samples show more than 4 copies of PIK3CA. (d) The change in the gene copy number of PIK3CA in breast cancer cells using real-time PCR. Representative images of fluorescence in situ hybridization (FISH) analysis in various breast cancer cell lines. Green signals represent bacterial artificial chromosome (BAC) 466H15 probe. Red signals represent chromosome 3 centromere probe.