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Figure 3 | Breast Cancer Research

Figure 3

From: Modulation of monocyte matrix metalloproteinase-2 by breast adenocarcinoma cells

Figure 3

MCF-7 and MDA-MB-231 upregulation of TIMP-1 and TIMP-2 protein levels from THP-1 monocytes. Immunoblots representative of three independent experiments carried out in duplicate using monoclonal (a,b) TIMP-1 and (c,d) TIMP-2 antibodies to show an increased production of both proteins by monocyte cells in response to MCF-7 and MDA-MB-231 CM. Lane 1 contains CM from THP-1 cells in serum-free media grown in the presence of 300 μl concentrated (a,c) MCF-7 CM and (b,d) MDA-MB-231 CM. Lane 2 contains CM from THP-1 cells in serum-free media grown in the presence of 300 μl concentrated serum-free media. Lane 3 contains serum-free media with 300 μl of (a,c) MCF-7 CM and (b,d) MDA-MB-231 CM in the absence of THP-1 cells. Lane 4 contains unconcentrated (a) MCF-7 and (b,d) MDA-MB-231 CM.

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