Figure 4From: Prime–boost vaccination with plasmid and adenovirus gene vaccines control HER2/neu+metastatic breast cancer in miceSurvival in a therapeutic metastasis model after vaccination with SINCP (prime) and Adeno (boost). Mice were given injections into the tail vein of A2L2 cells on day 0. On day 2, the mice were vaccinated with either SINCP-neu (a plasmid containing Sindbis virus genes and the gene for rat HER2/neu) or SINCP-βgal and on days 9 and 16 the mice were vaccinated with either Adeno-neu (an E1,E2a-deleted adenovirus also containing the gene for rat HER2/neu) or Adeno-empty (adenovirus lacking an inserted gene). (a) Overall survival of mice vaccinated with SINCP-neu and Adeno-neu compared with mice vaccinated with SINCP-βgal and Adeno-empty. (b) Overall survival of mice vaccinated with SINCP-neu and Adeno-neu compared with mice vaccinated with SINCP-βgal and Adeno-neu. P values were determined by a log-rank test of Kaplan–Meier survival curves.Back to article page