Figure 3

Flow cytometric analysis, using A2L2 cells, of serum from mice vaccinated with SINCP or Adeno. Serum was collected 2 weeks after the third vaccination of mice with SINCP-neu (a plasmid containing Sindbis virus genes and the gene for rat HER2/neu) or SINCP-βgal. (a) or 6 weeks after a single vaccination with Adeno-neu (an E1,E2a-deleted adenovirus also containing the gene for rat HER2/neu) or Adeno-empty (adenovirus lacking an inserted gene) (b). The immune serum was diluted with PBS (1:100), and fluorescein-isothiocyanate-labeled goat antimouse IgG diluted in PBS (1:1000) was used as the secondary antibody.