Figure 7From: Alternative initiation and splicing in dicer gene expression in human breast cellsVerification of Renilla luciferase mRNA levels by RT-PCR in transfected BT549 cells. β-Actin mRNA levels were used as control. Lane M, 1 kb plus DNA ladder (Invitrogen; lowest band is 200 bp); lane 1–3, RNA isolated from BT549 cells transfected with pcDNA3.1(+), pcDNA3.1(+)-short form 5'-leader or pcDNA3.1(+)-long form plasmid DNA, respectively.Back to article page