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Figure 7 | Breast Cancer Research

Figure 7

From: Connexin 43 mediated gap junctional communication enhances breast tumor cell diapedesis in culture

Figure 7

Non-functional Connexin43 (Cx43) does not enhance tumor cell diapedesis. HBL100 cells were transfected with Cx43 to which green fluorescent protein (GFP) was fused at the amino terminus (GFP-Cx43) to make it non-functional. Diapedesis of HBL100v cells and cells expressing functional (HBL100Cx43) or non-functional (HBL100GFP-Cx43) Cx43 was scored following co-culture with human microvascular endothelial cell (HMVEC) monolayers for 7 h. Analysis revealed that HBL100 cells transfected with GFP-Cx43 had an efficiency of diapedesis similar to HBL100v control cells in contrast to HBL100Cx43 cells, which showed significantly greater numbers of migrating cells. Data are expressed as the mean percentage ± SEM of five independent experiments in duplicate or triplicate (* p < 0.05).

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