Figure 5From: Connexin 43 mediated gap junctional communication enhances breast tumor cell diapedesis in cultureConnexin43 (Cx43) expression does not affect tumor cell adhesion to the endothelium. HBL100, HBL100v and Cx43 expressing HBL100 cells (HBL100Cx43) were labeled with dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine percholate (DiI), added for 1 h to endothelial monolayers and co-cultures were washed to remove non-adherent cells. Fluorescence intensity measurements revealed no significant difference in the number of tumor cells attached to the endothelium regardless of cell type. Data are expressed as mean percentage ± SEM of three independent experiments with eight replicates.Back to article page