Figure 8
In vivo inhibition of angiogenesis and increase in necrosis with celecoxib treatment. Vascularity of tumor implants was histologically evaluated by Masson's trichrome and factor VIII related antigen staining. Shown is evidence of central necrosis and decreased number of blood vessels in (b) a section of celecoxib-treated tumors relative to (a) a section obtained from a vehicle-treated animal (magnification 50×). Greater magnification (100×) of (c) panel a and (d) panel b are shown in the next two panels. Arrows in panel c point to blood vessels. Endothelial cells lining the blood vessels stained positively for factor VIII related antigen and showed larger blood vessels in the (e) vehicle-treated than in the (f) celecoxib-treated samples (magnification 100×).