Muscarinic receptors participation in angiogenic response induced by macrophages from mammary adenocarcinoma-bearing mice

Table 2 Participation of arginase and COX in angiogenesis induced by TMps

Treatment	Angiogenic response (vessels/mm²)	n
None	2.34 ± 0.06	5
CARB	4.96 ± 0.41*	5
NOHA + CARB	2.4 ± 0.6	4
INDO + CARB	1.9 ± 0.92	8
NS-398 + CARB	1.3 ± 0.31	5

Peritoneal macrophages (3 × 10⁵) from 7-day LMM3 tumor-bearing mice (TMps) stimulated with carbachol (CARB; 100 nM) in the absence or presence of N ^ω-hydroxy-L-arginine (NOHA; 100 μM), indomethacin (INDO; 1 μM) or NS-398 (10 μM) were inoculated intradermally into normal mice to evaluate the angiogenic response. *P < 0.001 compared with control (without treatment) (Mann–Whitney test). n, number of sites evaluated. Results are means ± SEM for three experiments.

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