Figure 3
Western blot assay to detect vascular endothelial growth factor (VEGF) expression. (a) Expression in normal macrophages (NMps) and macrophages from 7-day LMM3 tumor-bearing mice (TMps). (b) TMps were treated with carbachol (CARB) (100 nM) in the absence or presence of 1 μM pirenzepine (PIR), methoctramine (MET) or 4-DAMP. Lane C, control (TMps without treatment). Bands were quantified in optical density units per square millimeter (OD/mm2). The molecular mass indicated at the left corresponds to the VEGF isoform detected. One representative experiment of three is shown.