Paternal programming of breast cancer risk in daughters in a rat model: opposing effects of animal- and plant-based high-fat diets
© The Author(s). 2016
Received: 20 January 2016
Accepted: 17 June 2016
Published: 26 July 2016
Although males contribute half of the embryo’s genome, only recently has interest begun to be directed toward the potential impact of paternal experiences on the health of offspring. While there is evidence that paternal malnutrition may increase offspring susceptibility to metabolic diseases, the influence of paternal factors on a daughter’s breast cancer risk has been examined in few studies.
Male Sprague-Dawley rats were fed, before and during puberty, either a lard-based (high in saturated fats) or a corn oil-based (high in n-6 polyunsaturated fats) high-fat diet (60 % of fat-derived energy). Control animals were fed an AIN-93G control diet (16 % of fat-derived energy). Their 50-day-old female offspring fed only a commercial diet were subjected to the classical model of mammary carcinogenesis based on 7,12-dimethylbenz[a]anthracene initiation, and mammary tumor development was evaluated. Sperm cells and mammary gland tissue were subjected to cellular and molecular analysis.
Compared with female offspring of control diet-fed male rats, offspring of lard-fed male rats did not differ in tumor latency, growth, or multiplicity. However, female offspring of lard-fed male rats had increased elongation of the mammary epithelial tree, number of terminal end buds, and tumor incidence compared with both female offspring of control diet-fed and corn oil-fed male rats. Compared with female offspring of control diet-fed male rats, female offspring of corn oil-fed male rats showed decreased tumor growth but no difference regarding tumor incidence, latency, or multiplicity. Additionally, female offspring of corn oil-fed male rats had longer tumor latency as well as decreased tumor growth and multiplicity compared with female offspring of lard-fed male rats. Paternal consumption of animal- or plant-based high-fat diets elicited opposing effects, with lard rich in saturated fatty acids increasing breast cancer risk in offspring and corn oil rich in n-6 polyunsaturated fatty acids decreasing it. These effects could be linked to alterations in microRNA expression in fathers’ sperm and their daughters’ mammary glands, and to modifications in breast cancer-related protein expression in this tissue.
Our findings highlight the importance of paternal nutrition in affecting future generations’ risk of developing breast cancer.
KeywordsPaternal diet Breast cancer High-fat diet Female offspring
Breast cancer is a global public health problem, with nearly 1.7 million new cases diagnosed in 2012, representing 25 % of all cancers in women worldwide . Its incidence is projected to rise significantly over the next 20 years despite current efforts to prevent the disease . Although the precise reason for this growth is still not clear, it has been suggested that modern women’s lifestyles, including postponing first pregnancy and having fewer children, can explain the increase .
Nutritional habits, such as adoption of Western dietary patterns, are also linked to increased breast cancer risk . These patterns are characterized by low consumption of fruits and vegetables, increased energy intake, and decreased energy expenditure, leading to obesity, as well as increased intake of saturated fatty acids (SFA), n-6 polyunsaturated fatty acids (PUFA), and trans-fatty acids and decreased intake of n-3 polyunsaturated fats [5, 6]. While the majority of epidemiological studies on nutrition and breast cancer risk have been focused on women’s diet in adulthood, accumulating epidemiological and experimental evidence highlights early life experiences, including nutrition, as relevant factors for later breast cancer risk determination . The developmental origins of this cancer have been considered predominantly from a maternal perspective, with emphasis placed on the impact of high fat or energy intake during gestation and lactation on female offspring mammary gland development and later breast cancer risk [8, 9].
Although males contribute half of the embryo’s genome, only recently has interest begun to be directed toward the potential impact of paternal experiences on the health of offspring . While experimental studies have shown that paternal malnutrition may increase the susceptibility of offspring to metabolic dysregulation, obesity, and cardiovascular diseases [11, 12], the influence of paternal factors on daughter’s breast cancer risk has been examined in few studies. Among them, epidemiological studies show an association between higher paternal education level, older age, and smoking with increased rate of breast cancer in the daughters [13, 14].
Unlike the female production of germ cells that takes place predominantly in early life , male production of germ cells starts in utero, with mature sperm cells being produced throughout the entire reproductive life of the male . Because spermatogenesis can be dramatically influenced by environmental factors, including malnutrition, obesity, and an exposure to toxic compounds, the father’s health during preconception is now acknowledged as a critical factor in the context of the developmental origins of health and disease . In addition to embryogenesis, gametogenesis comprises intense epigenetic (DNA methylation, histone modification, and microRNA [miRNA or miR] expression) remodeling [18, 19]. Thus, epigenetically inherited increased disease risk could be transmitted through the female as well as the male germline . Specific windows within which male gametes would be especially prone to environmentally elicited epigenetic deregulation include prepuberty and the reproductive phase .
Given the marked increase in dietary fat intake over the past three decades , as well as the notion that different kinds of dietary fats can lead to different health outcomes , we designed this study to investigate whether, in rats, consumption of high levels of animal- or vegetable-based fats by fathers would affect their daughters’ risk of breast cancer. We also investigated the underlying cellular and molecular mechanisms. We fed male Sprague-Dawley rats, before and during puberty, either a lard-based (high in SFA) or a corn oil-based (high in n-6 PUFA) high-fat diet (60 % of fat-derived energy). Control animals were fed a control AIN-93G diet containing soybean oil as a fat source (16 % of fat-derived energy). Male rats were mated with female rats that were consuming a commercial diet. We show that paternal consumption of these high-fat diets elicited opposing effects, with animal fat increasing and vegetable oil decreasing breast cancer risk in the offspring. These effects could be linked to alterations in miRNA expression in fathers’ sperm and their daughters’ mammary glands, as well as to modifications in breast cancer-related protein expression in this tissue. These novel data show that paternal high-fat diets influence their female offspring’s susceptibility to mammary cancer, with consumption of lard increasing and corn oil reducing daughters’ mammary cancer risk. Thus, paternal diet before conception sets a stage for a daughter’s risk of developing breast cancer.
This study was approved by the ethics committee on animal experiments of the Faculty of Pharmaceutical Sciences, University of São Paulo (protocol number CEUA/FCF/381). Twenty-one-day-old male rats were divided into three groups (n = 20 rats per group): control rats (those fed the control AIN-93G diet, with 16 % of total calories provided by lipids), lard-fed males (exposed to a high-SFA diet, with 60 % of total calories provided mainly from lard), and corn oil-fed males (exposed to n-6 PUFA diet, with 60 % of total calories provided mainly from corn oil). At 12 weeks of age, all male rats were switched to a chow diet and mated by housing one male with one female per cage. Pregnant female rats and their offspring consumed only commercial laboratory chow (Nuvital Nutrientes, Colombo, Brazil). Body weight and food intake were recorded two or three times per week.
Determination of the diets’ lipid profiles
The lipid profiles of the control, lard, and corn oil diets were determined according to the methods published by the Association of Official Analytical Chemists . Fatty acids were esterified to fatty-acid methyl esters according to the method reported by Hartman and Lago , and their composition was analyzed with a gas chromatograph (GC 17A/Class GC 10; Shimadzu, Kyoto, Japan) equipped with a flame ionization detector and a SUPELCOWAX® 10 fused silica capillary column (30 mm × 0.25 mm inner diameter; Sigma-Aldrich, St. Louis, MO, USA). The temperature was set at 170 °C, raised to 225 °C at a rate of 1 °C/minute, and held for 25 minutes. The temperatures of the vaporizer and detector were 250 °C and 270 °C, respectively. Helium was used as the carrier gas (1 ml/minute). Identification of the fatty acids was performed by comparison of the retention times with the standard mixture of fatty-acid methyl esters. Each determination was performed in duplicate using two different samples for each diet.
Insulin tolerance test
The tests were performed at 0800 h after the rats were fasted for 12 h, according to the method described by Takada et al. . The insulin load (75 mU/100 g body weight) was injected as a bolus, and the blood glucose levels were determined at 0, 3, 6, 9, 12, and 30 minutes after injection in male rats and their 50-day-old female offspring. The AUC was calculated according to the trapezoid rule .
Mature spermatozoa collection and purification
Control diet and lard- and corn oil-fed male rats were killed once females were pregnant, and the caudal epididymis was dissected for sperm collection. The cauda and vas deferens from male rats were collected, punctured, and transferred to tissue culture dishes containing M2 medium (M2 medium with HEPES, without penicillin and streptomycin, sterile-filtered, suitable for rat embryo; Sigma-Aldrich), where it was incubated for 1 h at 37 °C. Spermatozoa samples were washed with PBS and then incubated with somatic cell lysis buffer (SCLB; 0.1 % SDS, 0.5 % Triton X-100 in diethylpyrocarbonate water) for 1 h, according to a protocol described by Goodrich et al. . SCLB was rinsed off with two washes of PBS, and the purified spermatozoa sample (at least 95 % purity as assessed by microscopy) was pelleted and used for miRNA extraction.
Determination of daily sperm production
The right testis was maintained at −20 °C until processing to determine the daily sperm production. The technique proposed by Robb et al.  is based on the resistance of elongated spermatids present in phases 17–19 of spermatogenesis to intense mechanical stress due to the high compaction of chromatin.
Sperm morphological analyses
According to the method of Seed et al. , the epididymis was previously frozen at −20 °C, underwent incision, and was subsequently immersed in PBS to promote the dissemination of gametes to the aqueous medium. Then the obtained solution was placed on slides for examination by light microscopy. Two hundred sperm per animal were analyzed microscopically at × 400 magnification.
Mammary gland harvesting
Abdominal mammary glands of female offspring of control diet- and lard- and corn oil-fed male rats (n = 6 per group) were collected on postnatal day 50 and used for preparing mammary whole mounts and miRNA and protein extraction.
Analysis of mammary gland morphology and development
Whole-mount preparations of the fourth abdominal mammary gland from 50-day-old female offspring (n = 5/group) were obtained, and the epithelial elongation and number of terminal end buds (TEBs) were determined as described by de Assis et al. .
Mammary tumor induction
Mammary tumors were induced in 50-day-old female rat offspring (n = 24 rats/group) by administration of 7,12-dimethylbenz[a]anthracene (DMBA, 50 mg/kg body weight; Sigma-Aldrich). The carcinogen was dissolved in corn oil and administered by oral gavage. Animals were examined for mammary tumors by palpation twice per week. Latency of tumor appearance, the number of animals with tumors, and the number of tumors per animal (multiplicity) were evaluated. The tumor volume was calculated with tumor measures of length (a), height (b), and width (c) taken with a caliper rule once per week since tumor appearance and throughout the experiment. The formula (1/6 × 3.14) × (a × b × c) was used to calculate the tumor volume, as described by Spang-Thomsen et al. . The tumor growth rate was calculated using the measured volumes of each tumor at a given week (d) and the subsequent week (e) of appearance using the formula [(e − d)/d] × 100. Those animals in which tumor burden approximated 10 % of total body weight were killed. All others animals were killed 19 weeks after carcinogen administration.
Analysis of mammary gland and tumor cell proliferation and apoptosis in female offspring
Cell proliferation was evaluated in mammary glands (ducts and lobules) and tumors from 50-day-old female offspring (n = 4/group) by Ki-67 immunohistochemistry. After being harvested, mammary tissue was directly fixed in 10 % buffered formalin, embedded in paraffin, and sectioned. Sections were then deparaffinized in xylene and hydrated through graded ethanol. Antigen retrieval was performed with 10 mM citrate buffer, pH 6, for 20 minutes in a pressure cooker. Peroxidase blocking was performed with 10 % H2O2 for 10 minutes, and nonspecific binding was blocked for 1 h with 1 % skimmed milk in PBS. Sections were incubated overnight with anti-rat Ki-67 primary antibody (Abcam, Cambridge, UK) at a 1:50 dilution. After washes, sections were incubated with the LSAB 2 System-HRP kit (Dako, Carpinteria, CA, USA) according to the manufacturer’s instructions, stained with 3,3′-diaminobenzidine in chromogenic solution (Dako) for 10 minutes, washed, and counterstained for 1.5 minutes with hematoxylin. Cell proliferation was quantified by assessing the number of Ki-67-positive cells among 1000 cells. The slides were evaluated using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Apoptosis analysis was conducted in mammary glands (ducts and lobules) and tumors from 50-day-old female offspring (n = 4/group), according to the method described by Elmore et al. , using ImageJ software. Results are presented as mean number of apoptotic cells per 1000 cells.
microRNA expression profile analysis
Total RNA from paternal sperm and their female offspring’s total mammary gland was extracted using the miRNeasy Mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. RNA samples were quantified and stored at −80 °C until use. miRNA arrays were performed at the Genomics and Epigenomics Shared Resources at Georgetown University using Applied Biosystems TaqMan Array Rodent MicroRNA arrays (Life Technologies, Carlsbad, CA, USA) to generate the miRNA expression profiles for each experimental group. The TaqMan® Array Rodent MicroRNA A + B Cards Set v3.0 is a two-card set containing a total of 384 TaqMan® MicroRNA assays per card (Life Technologies). The set enables accurate quantitation of 641 and 373 unique miRNAs for rat. There are three TaqMan® MicroRNA Assay endogenous controls for each species on each array to aid in data normalization . The geNorm algorithm was applied to those endogenous controls to determine the optimal number of stable controls. The geometric mean of these selected controls was used for array normalization. To conduct further statistical analysis, the normalized value was log-transformed to meet the t test requirement. Statistical analysis was conducted using the limma package in R . miRNAs that had a false discovery rate <0.1 were considered as significantly altered and selected for further analysis. Target prediction for miRNAs of interest was conducted using TargetScan (release 6.2). The predicted targeted messenger RNA (mRNA) list was then uploaded to Ingenuity Pathway Analysis (IPA; QIAGEN Silicon Valley, Redwood City, CA, USA) for gene set enrichment analysis. We selected the top canonical pathways for further analysis.
Analysis of protein levels in mammary glands of female offspring
Protein levels of 50 ng/μl were assessed by Western blot analysis in total mammary glands obtained from 50-day-old female rats (n = 5 per group). Total protein was extracted from mammary tissues using radioimmunoprecipitation assay buffer with protease inhibitor (Roche, Basel, Switzerland), glycerophosphate (10 mM), sodium orthovanadate (1 mM), pyrophosphate (5 mM), and phenylmethylsulfonyl fluoride (1 mM). The samples were mixed with the buffer for 5 minutes, then incubated on ice for 30 minutes and centrifuged for 10 minutes at high speed. Protein in the supernatant was quantified using Pierce bicinchoninic acid protein assay reagent (Thermo Scientific, Rockford, IL, USA). Protein extracts were resolved on a 4–12 % gradient denaturing polyacrylamide gel (SDS-PAGE). Proteins were transferred using the Invitrogen iBlot® 7-Minute Dry Blotting System (Life Technologies) and blocked with 5 % nonfat dry milk for 1 h at room temperature. Membranes were incubated with the specific primary antibodies at 4 °C overnight. After several washes, the membranes were incubated with HRP-conjugated secondary antibody (1:5000; Santa Cruz Biotechnology, Dallas, TX, USA) at room temperature for 1 h. Membranes were developed using HyGLO chemiluminescent HRP antibody detection reagent (Denville Scientific Inc., Metuchen, NJ, USA), and exposed to Kodak autoradiography films (Carestream Health, Rochester, NY, USA). The optical density of the bands was quantified using Quantity One software (Bio-Rad Laboratories, Hercules, CA, USA). To control for equal protein loading, expression of the proteins of interest was normalized to the β-actin signal.
Results are expressed as the mean ± SEM, and all analyses were conducted with the limma package in R. Multiple-group comparisons were performed using one-way analysis of variance (ANOVA) followed by a least significant difference (LSD) test, and two-group comparisons were performed using Student’s t test. Repeated-measures ANOVA was applied for caloric intake data evaluation, and Kaplan-Meier curves and log-rank tests were applied for determining differences in tumor incidence. For all data analyses, p ≤ 0.05 was applied as the threshold for statistical significance.
Paternal dietary and health parameters
Health parameters of male rats and their 50-day-old female offspring in control diet, lard-based diet, and corn oil-based diet groups
363.1 ± 6.4 g
398.2 ± 12.0 ga
369.4 ± 7.8 gb
1.4 ± 0.1 g/100 g body weight
2.7 ± 0.2 g/100 g body weighta
2.6 ± 0.2 g/100 g body weighta
0.8 ± 0.1 g/100 g body weight
1.9 ± 0.1 g/100 g body weighta
1.7 ± 0.2 g/100 g body weighta
1.2 ± 0.1 g/100 g body weight
2.7 ± 0.1 g/100 g body weighta
2.0 ± 0.2 g/100 g body weighta,b
0.45 ± 0.01 g/100 g body weight
0.40 ± 0.01 g/100 g body weighta
0.45 ± 0.01 g/100 g body weightb
0.14 ± 0.00 g/100 g body weight
0.13 ± 0.00 g/100 g body weighta
0.14 ± 0.00 g/100 g body weightb
0.33 ± 0.01 g/100 g body weight
0.29 ± 0.02 g/100 g body weightc
0.33 ± 0.02 g/100 g body weightd
Sperm morphology (% normal)
66.4 ± 2.8 %
50.1 ± 3.0 %a
70.9 ± 2.9 %b
Daily sperm production, n/testicle/day
31 ± 1.9 × 106
23 ± 0.9a × 106
29 ± 0.8b × 106
100.8 ± 3 mg/dl
124.6 ± 4 mg/dla
116.1 ± 3 mg/dla
50-day-old female offspring
8.0 ± 1.5 g
8.8 ± 1.0 ga
9.1 ± 1.5 ga
138.9 ± 1.5 g
147.0 ± 1.2 ga
142.1 ± 1.1 gb,c
0.9 ± 0.1 g/100 g body weight
1.1 ± 0.0 g/100 g body weighta
1.2 ± 0.1 g/100 g body weighta
106.4 ± 2.0 mg/dl
112.5 ± 1.9 mg/dla
106.3 ± 1.2 mg/dlb
Female offspring health parameters
Female offspring of both lard- and corn oil-fed male rats had greater birth weight (p ≤ 0.05) and greater weight gain (p ≤ 0.05; p ≤ 0.08 for offspring of corn oil-fed male rats) than offspring of control diet-fed male rats (Table 1). There was no difference (p > 0.05) between female offspring of lard- and corn oil-fed male rats regarding birth weight (Table 1). Female offspring of corn oil-fed male rats had less (p ≤ 0.05) weight gain than offspring of lard-fed male rats (Table 1). Similarly to the male rats, female offspring of both the lard-fed and the corn oil-fed male rats had greater (p ≤ 0.05) retroperitoneal fat weights than offspring of control diet-fed male rats (Table 1). There was no difference (p > 0.05) between female offspring of lard- or corn oil-fed male rats regarding this parameter (Fig. 1c). Female offspring of lard-fed male rats had higher (p ≤ 0.05) fasting glucose levels (Table 1) and higher (p ≤ 0.05) AUCs than female offspring of control diet- or corn oil-fed male rats (Fig. 1c). There was no difference (p > 0.05) between female offspring of control diet- and corn oil-fed male rats regarding these parameters (Table 1 and Fig. 1c).
Female offspring mammary gland morphology
Female offspring mammary gland tumors data
Female offspring mammary gland and tumor cell proliferation and apoptosis
miRNA expression profile in fathers’ sperm cells and in their daughters’ mammary glands
Protein expression in female offspring mammary gland
We further explored if Tgfβ and key regulators of its activity were altered by measuring protein levels of v-akt murine thymoma viral oncogene (Akt), cofilin (Cfl), v-raf leukemia viral oncogene (c-Raf), extracellular signal-regulated kinase 1/2 (Erk1/2), phosphorylated mitogen-activated protein kinase 8 (p-Jnk), mitogen-activated protein kinase 4 (Mkk4), mechanistic target of rapamycin (Mtor), mitogen-activated protein kinase 14 (p38), phosphorylated Smad family member 3/Smad family member 3 (p-Smad3/Smad3) ratio, and Harvey rat sarcoma virus oncogene (Ras). Tgfβ protein expression was higher (p ≤ 0.05) in the mammary glands of the female offspring of lard-fed male rats than in the offspring of control diet- and corn oil-fed male rats. There was no difference (p > 0.05) between female offspring of corn oil- and control diet-fed male rats regarding this protein (Fig. 6). Further, the levels of Akt and p-Jnk were higher (p ≤ 0.05) in the female offspring of lard-fed male rats than in female offspring of control diet-fed male rats (Fig. 6). There was no difference (p > 0.05) in female offspring of corn oil-fed male rats and the offspring of control diet- and lard-fed male rats regarding these proteins (Fig. 6). Female offspring of corn oil-fed male rats had lower levels of Mtor, Mkk4 (p ≤ 0.06), and p-Smad3/Smad3 (p ≤ 0.05) than female offspring of lard-fed, but not control diet-fed, male rats (Fig. 6). There was no difference (p > 0.05) between female offspring of control diet- and lard-fed male rats regarding these proteins (Fig. 6). In addition to promoting EMT, all these altered proteins are involved in increasing cell survival, growth, migration, and invasion.
Breast cancer is a complex disease with a multifactorial etiology . It is increasingly evident that in utero environment can program later susceptibility to breast cancer . The findings of our present study suggest that breast cancer risk can be determined even earlier through diet-induced changes in paternal germ cells before conception. Our study shows that, compared with female offspring of control diet-fed fathers, offspring of lard-fed fathers did not differ in tumor latency, growth, or multiplicity. However, female offspring of lard-fed fathers had increased elongation of the mammary epithelial tree, number of TEBs, and tumor incidence compared with both control diet- and corn oil-fed fathers, showing that paternal exposure to a lard-based high-fat diet containing SFA increased their daughters’ mammary cancer risk. TEBs are considered sites of tumor initiation , and increased epithelial elongation reflects rapid epithelial growth . Additionally, female offspring of lard-fed fathers showed increased cell proliferation and decreased apoptosis in the mammary gland lobules compared with female offspring of both control diet- and corn oil-fed fathers. Altogether, these findings support the view that altered mammary gland development represents a potential underlying mechanism of increased breast cancer risk .
Compared with female offspring of control diet-fed fathers, female offspring of corn oil-fed fathers had decreased tumor growth. There was no difference in tumor incidence, latency, or multiplicity between female offspring of control diet- and corn oil-fed fathers. In addition, female offspring of corn oil-fed fathers had longer tumor latency, decreased tumor growth, and decreased multiplicity compared with female offspring of lard-fed fathers. These data show that paternal exposure to a corn oil-based high-fat diet containing n-6 PUFA had an effect opposite that of a lard-based high-fat diet and reduced their daughters’ mammary cancer risk.
Although male rats that were fed the lard-based and corn oil-based high-fat diets consumed the same amount of calories, lard increased the body weight and size of epididymal fat pads more than corn oil did. Thus, different fatty acids can have distinct effects on adipose accumulation, as already shown by others . Our results further show that lard, but not corn oil, elicited detrimental effects on male reproductive parameters (fewer normal sperm cells and lower daily sperm production). This is in line with earlier human and animal data showing that SFA disrupt testicular metabolism and sperm quality, while PUFA are essential for sperm cell membrane fluidity and flexibility as well as fertilization . Excessive epididymal fat in lard-fed males may have been detrimental to spermatogenesis, as epididymal tissue is an essential depot for spermatogenesis . The adverse effects of lard may not be mediated through increased insulin resistance. Although a correlation between insulin resistance and impaired sperm production has been reported in rats fed a diet high in SFA , as also found in the present study, a corn oil-based high-fat diet also impaired insulin tolerance but did not affect male reproductive parameters. We propose that impaired sperm quality and function in lard-fed fathers could be associated with disruption in metabolic programming and increased breast cancer risk among their daughters.
The impact of obesity in fathers leading to metabolic dysfunction in their female offspring was previously observed in rodent studies , and it was also seen in our present study. Female offspring of both lard- and corn oil-fed fathers exhibited increased body weight and adiposity. However, only female offspring of lard-fed fathers displayed an impaired insulin response, indicating that the type of dietary fatty acids consumed represents a key factor in metabolic programming through the male germline.
Epigenetic modifications that are necessary for achieving reproductive capacity of male gametes include DNA methylation, histone retention, and expression of noncoding RNAs such as miRNAs . Because miRNAs can modulate the expression of hundreds of mRNAs that affect embryonic development as well as the establishment of the offspring’s epigenome , they have been proposed to mediate paternal programming effects on the offspring . The epididymis has been implicated as the site of the alterations of miRNA signatures occurring during the maturation of sperm cells, and therefore an increase in epididymal fat pad size could potentially impact inheritance of miRNA signatures and/or the developmental trajectory of the offspring . The impact of high-fat-diet-induced male obesity on the miRNA profile in mature spermatozoa has been examined in rodent studies . In a study by Fullston et al. , males fed a high-saturated-fat diet exhibited changes in miRNAs in the testes and mature spermatozoa that target mRNA associated with spermatogenesis, embryonic development, and metabolic diseases in the offspring. We provide further evidence that paternal nutrition can impact the sperm miRNA profile and possibly the subsequent mammary gland miRNA profile, which in turn targets genes implicated in breast cancer and other diseases.
Some of the miRNAs that were differentially expressed in the lard- and corn oil-fed fathers’ germ cells also were differentially expressed in their daughters’ mammary glands, although the daughters were never directly exposed to the high-fat diets. When we compared lard-fed fathers and their daughters, we observed three miRNA that were significantly altered in both the sperm and mammary glands of corn oil-fed fathers and their daughters: miR-1897-5p, miR-219-1-3p, and miR-376a#. Since miRNAs can modulate gene expression by inhibiting the translation of mRNA or by directing their degradation , we focused on determining if the expression of the top potentially targeted proteins (Additional file 1: Table S1) was altered in the daughters of lard- or corn oil-fed fathers. Among them, we highlight Pkd1 and Tgfβr1. PKD1 is a serine/threonine kinase that is expressed in ductal epithelial cells of the mammary gland, maintains the epithelial phenotype, and prevents EMT . Inhibition of PKD1 can lead to pathological conditions such as cancer . Thus, our finding of increased Pkd1 levels in the mammary glands of corn oil-fed fathers’ offspring, compared with female offspring of lard-fed fathers, is in line with their lowest susceptibility to breast cancer. In addition, compared with female offspring of control diet- and corn oil-fed fathers, another miRNA target, Tgfβr1, was increased in the daughters of lard-fed fathers that displayed the highest susceptibility to mammary cancer. Tgfβr1 expression is related to promotion of breast carcinogenesis through multiple mechanisms, including enhancing EMT . We assessed the protein levels of up- and downstream signaling partners of Tgfβr1. Female offspring of lard-fed fathers showed higher protein levels of Tgfβ than female offspring of both control diet- and corn oil-fed fathers, as well as higher protein levels of Akt and p-Jnk than control diet-fed fathers. In addition, female offspring of corn oil-fed rats had lower levels of Mtor, Mkk4, and p-Smad3/Smad3 than female offspring of lard-fed fathers. These proteins collectively play important roles in cell survival, growth, migration, and invasion [57, 58]. These findings indicate that mechanisms other than miRNAs contribute to changes in gene expression in the daughters’ mammary tissue following paternal exposure to a lard-based high-fat diet.
Because fathers, mothers, and their daughters tend to share the same nutritional habits , it is important to further investigate if paternally programmed breast cancer risk is affected by maternal and female offspring’s fat intake. Maternal intake of a high corn oil diet during pregnancy increases female offspring’s mammary cancer risk , while intake of lard has opposite effects . In addition, because obesity-induced altered sperm miRNA expression in the fathers can be normalized through exercise or dietary intervention (consumption of balanced diet), which then improves the metabolic health of female offspring , the efficacy of a similar intervention in reducing daughters’ breast cancer risk should be investigated.
In the present study, we show that paternal intake of a lard-based high-fat diet rich in SFA increases female offspring’s mammary cancer risk, as indicated by the increased elongation of the mammary epithelial tree, number of TEBs, and tumor incidence in female offspring of lard-fed fathers compared with female offspring of both control diet- and corn oil-fed rats. However, if the paternal fat source is corn oil that is high in n-6 PUFA, these male rats’ offspring’s mammary cancer risk is reduced, as indicated by decreased tumor growth in female offspring of corn oil-fed fathers compared with female offspring of both control diet- and lard-fed fathers, as well as by longer tumor latency and decreased tumor multiplicity compared with female offspring of lard-fed fathers. Altered miRNA expression in fathers’ sperm and daughters’ mammary glands may at least underlie these effects, but other epigenetic changes are likely to be involved. Our findings highlight the importance of paternal nutrition in affecting future generations’ risk of developing breast cancer.
Akt, v-akt murine thymoma viral oncogene; ANOVA, analysis of variance; Casp3, caspase 3; CB, rats that were fed a corn oil-based high-fat diet and their offspring; Cebpβ, CCAAT/enhancer-binding protein beta; Cfl, cofilin; CO, rats that were fed a control diet and their offspring; c-Raf, v-raf leukemia viral oncogene; DMBA, 7,12-dimethylbenz[a]anthracene; EMT, epithelial-to-mesenchymal transition; Erk1/2, extracellular signal-regulated kinase 1/2; Igf1r, insulin-like growth factor 1 receptor; ITT, insulin tolerance test; LB, rats that were fed a lard-based high-fat diet and their offspring; LSD, least significant difference; miRNA or miR, microRNA; Mkk4, mitogen-activated protein kinase kinase 4; mRNA, messenger RNA; Mtor, mechanistic target of rapamycin; MUFA, monounsaturated fatty acid; p38, mitogen-activated protein kinase 14; p-Jnk, phosphorylated mitogen-activated protein kinase 8; Pkd1, protein kinase D1; p-Smad3/Smad3, phosphorylated Smad family member 3/Smad family member 3 ratio; PTEN, phosphatase and tensin homolog; PUFA, polyunsaturated fatty acid; Ras, Harvey rat sarcoma virus oncogene; SCLB, somatic cell lysis buffer; SFA, saturated fatty acid; TEB, terminal end bud; Tgfβr1, transforming growth factor, beta receptor I
We thank the following Georgetown Lombardi Cancer Center Shared Resources (SR) for their assistance: Animal Model SR, Histopathology & Tissue SR, and Genomics & Epigenomics SR. We also thank Luciane F. L. Macedo (Faculty of Pharmaceutical Sciences, University of São Paulo) for technical assistance with the dietary fatty acid quantification, Prof. Odair Aguiar Jr. (Federal University of São Paulo) for technical support with the male reproductive parameters analysis, Prof. Luis Fernando Barbisan (Institute of Biosciences, State University of São Paulo) for technical support with cell apoptosis analysis, and Prof. Fernando Salvador Moreno (Faculty of Pharmaceutical Sciences, University of São Paulo) for technical support with the rat experimental protocol.
This study was supported by the Brazilian National Council for Scientific and Technological Development (CNPq; process 153478/2012-8), the São Paulo State Research Funding Agency (FAPESP; process 2011/23259-4), the National Cancer Institute (grant R01 CA164384 to LHC), and the Prevent Cancer Foundation (research grant 299045 to SdA).
CCF conceived of the study; supervised animal work, tissue collection, and molecular analysis; and drafted the manuscript. LNG assisted with animal studies, tissue collection, and molecular assays and helped to revise the manuscript. MPR assisted with animal studies, tissue collection, and molecular assays and helped to revise the manuscript. FdOA assisted with animal study design and data analyses and helped to revise the manuscript. LJ performed miRNA data analysis and helped to revise the manuscript. JI assisted with tumor and statistical analyses and helped to revise the manuscript. VCP conducted male reproductive parameter analysis and helped to revise the manuscript. IAdC conducted analysis of the diets’ lipid profiles and helped to revise the manuscript. LHC provided intellectual input and helped to draft the manuscript. SdA provided intellectual input and helped to draft the manuscript. TPO conceived of the study, oversaw the research, and drafted the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing financial or non-financial competing interests.
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- Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. GLOBOCAN 2012 v1.0: estimated cancer incidence, mortality and prevalence worldwide in 2012. Lyon, France: International Agency for Research on Cancer; 2013. http://globocan.iarc.fr. Accessed 17 Nov 2015.
- Rahib L, Smith BD, Aizenberg R, Rosenzweig AB, Fleshman JM, Matrisian LM. Projecting cancer incidence and deaths to 2030: the unexpected burden of thyroid, liver, and pancreas cancers in the United States. Cancer Res. 2014;74:2913–21.View ArticlePubMedGoogle Scholar
- Howell A, Anderson AS, Clarke RB, Duffy SW, Evans DG, Garcia-Closas M, Gescher AJ, Key TJ, Saxton JM, Harvie MN. Risk determination and prevention of breast cancer. Breast Cancer Res. 2014;16:446.
- Albuquerque RC, Baltar VT, Marchioni DM. Breast cancer and dietary patterns: a systematic review. Nutr Rev. 2014;72:1–17.View ArticlePubMedGoogle Scholar
- Cordain L, Eaton SB, Sebastian A, Mann N, Lindeberg S, Watkins BA, O'Keefe JH, Brand-Miller J. Origins and evolution of the Western diet: health implications for the 21st century. Am J Clin Nutr. 2005;81:341–54.
- Lawrence GD. Dietary fats and health: dietary recommendations in the context of scientific evidence. Adv Nutr. 2013;4:294–302.View ArticlePubMedPubMed CentralGoogle Scholar
- Colditz GA, Bohlke K, Berkey CS. Breast cancer risk accumulation starts early: prevention must also. Breast Cancer Res Treat. 2014;145:567–79.View ArticlePubMedPubMed CentralGoogle Scholar
- Hilakivi-Clarke L, Clarke R, Onojafe I, Raygada M, Cho E, Lippman M. A maternal diet high in n-6 polyunsaturated fats alters mammary gland development, puberty onset, and breast cancer risk among female rat offspring. Proc Natl Acad Sci U S A. 1997;94:9372–7.View ArticlePubMedPubMed CentralGoogle Scholar
- De Oliveira AF, Fontelles CC, Rosim MP, de Oliveira TF, de Melo Loureiro AP, Mancini-Filho J, Rogero MM, Moreno FS, de Assis S, Barbisan LF, Hilakivi-Clarke L, Ong TP. Exposure to lard-based high-fat diet during fetal and lactation periods modify breast cancer susceptibility in adulthood in rats. J Nutr Biochem. 2014;25:613–22.
- Ng SF, Lin RC, Laybutt DR, Barres R, Owens JA, Morris MJ. Chronic high-fat diet in fathers programs β-cell dysfunction in female rat offspring. Nature. 2010;467:963–6.View ArticlePubMedGoogle Scholar
- Watkins AJ, Sinclair KD. Paternal low protein diet affects adult offspring cardiovascular and metabolic function in mice. Am J Physiol Heart Circ Physiol. 2014;306:H1444–52.View ArticlePubMedGoogle Scholar
- Hughes V. Epigenetics: the sins of the father. Nature. 2014;507:22–4.View ArticlePubMedGoogle Scholar
- Titus-Ernstoff L, Egan KM, Newcomb PA, Ding J, Trentham-Dietz A, Greenberg ER, Baron JA, Trichopoulos D, Willett WC. Early life factors in relation to breast cancer risk in postmenopausal women. Cancer Epidemiol Biomarkers Prev. 2002;11:207–10.
- de Kok IM, van Lenthe FJ, Avendano M, Louwman M, Coebergh JW, Mackenbach JP. Childhood social class and cancer incidence: results of the GLOBE study. Soc Sci Med. 2008;66:1131–9.View ArticlePubMedGoogle Scholar
- Hanna CB, Hennebold JD. Ovarian germline stem cells: an unlimited source of oocytes? Fertil Steril. 2014;101:20–30.View ArticlePubMedPubMed CentralGoogle Scholar
- Romero Y, Calvel P, Nef S. Non-coding small RNAs and spermatogenesis. Med Sci. 2012;28:490–6.Google Scholar
- Godmann M, Lambrot R, Kimmins S. The dynamic epigenetic program in male germ cells: its role in spermatogenesis, testis cancer, and its response to the environment. Microsc Res Tech. 2009;72:603–19.View ArticlePubMedGoogle Scholar
- Gill ME, Erkek S, Peters AH. Parental epigenetic control of embryogenesis: a balance between inheritance and reprogramming? Curr Opin Cell Biol. 2012;24:387–96.View ArticlePubMedGoogle Scholar
- Dean W. DNA methylation and demethylation: a pathway to gametogenesis and development. Mol Reprod Dev. 2014;81:113–25.View ArticlePubMedGoogle Scholar
- Aiken CE, Ozanne SE. Transgenerational developmental programming. Hum Reprod Update. 2014;20:63–75.View ArticlePubMedGoogle Scholar
- Soubry A, Hoyo C, Jirtle RL, Murphy SK. A paternal environmental legacy: evidence for epigenetic inheritance through the male germ line. Bioessays. 2014;36:359–71.View ArticlePubMedPubMed CentralGoogle Scholar
- Varela-Moreiras G. Controlling obesity: what should be changed? Int J Vitam Nutr Res. 2006;76:262–8.View ArticlePubMedGoogle Scholar
- Nettleton JA, Jebb S, Risérus U, Koletzko B, Fleming J. Role of dietary fats in the prevention and treatment of the metabolic syndrome. Ann Nutr Metab. 2014;64:167–78.View ArticlePubMedGoogle Scholar
- Association of Official Analytical Chemists (AOAC). Fat (Total, Saturated and Unsaturated) in Foods. In: Horwitz W, editor. Official methods of analysis of AOAC International. Vol 2 Food composition; Additives; Natural contaminants. 17th edition. Rockville, MD, USA. Gaithersburg, Md: AOAC International; 2002. p. 20–4.
- Hartman L, Lago RC. Rapid preparation of fatty acid methyl esters from lipids. Lab Pract. 1973;22:45–6.Google Scholar
- Takada J, Fonseca-Alaniz MH, de Campos TB, Andreotti S, Campana AB, Okamoto M, Borges-Silva Cd, Machado UF, Lima FB. Metabolic recovery of adipose tissue is associated with improvement in insulin resistance in a model of experimental diabetes. J Endocrinol. 2008;198:51–60.
- Le Floch JP, Escuyer P, Baudin E, Baudon D, Perlemuter L. Blood glucose area under the curve: methodological aspects. Diabetes Care. 1990;13:172–5.View ArticlePubMedGoogle Scholar
- Goodrich R, Johnson G, Krawetz SA. The preparation of human spermatozoal RNA for clinical analysis. Arch Androl. 2007;53:161–7.View ArticlePubMedGoogle Scholar
- Robb GW, Amann RP, Killian GJ. Daily sperm production and epididymal sperm reserves of pubertal and adult rats. J Reprod Fertil. 1978;54:103–7.View ArticlePubMedGoogle Scholar
- Seed J, Chapin RE, Clegg ED, Dostal LA, Foote RH, Hurtt ME, et al. Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. ILSI Risk Science Institute Expert Working Group on Sperm Evaluation. Reprod Toxicol. 1996;10:237–44.View ArticlePubMedGoogle Scholar
- de Assis S, Warri A, Cruz MI, Hilakivi-Clarke L. Changes in mammary gland morphology and breast cancer risk in rats. J Vis Exp. 2010;44:2260.Google Scholar
- Spang-Thomsen M, Rygaard K, Hansen L, Halvorsen AC, Vindeløv LL, Brünner N. Growth kinetics of four human breast carcinomas grown in nude mice. Breast Cancer Res Treat. 1989;14:235–43.View ArticlePubMedGoogle Scholar
- Elmore SA, Dixon D, Hailey JR, Harada T, Herbert RA, Maronpot RR, Nolte T, Rehg JE, Rittinghausen S, Rosol TJ, Satoh H, Vidal JD, Willard-Mack CL, Creasy DM. Recommendations from the INHAND Apoptosis/Necrosis Working Group. Toxicol Pathol. 2016;44:173–88.
- Brattelid T, Aarnes EK, Helgeland E, Guvaåg S, Eichele H, Jonassen AK. Normalization strategy is critical for the outcome of miRNA expression analyses in the rat heart. Physiol Genomics. 2011;43:604–10.View ArticlePubMedGoogle Scholar
- Shen B, Zhou S, He Y, Zhao H, Mei M, Wu X. Revealing the underlying mechanism of ischemia reperfusion injury using bioinformatics approach. Kidney Blood Press Res. 2013;38:99–108.View ArticlePubMedGoogle Scholar
- Du C, Zhang C, Hassan S, Biswas MH, Balaji KC. Protein kinase D1 suppresses epithelial-to-mesenchymal transition through phosphorylation of Snail. Cancer Res. 2010;70:7810–9.View ArticlePubMedGoogle Scholar
- Wendt MK, Tian M, Schiemann WP. Deconstructing the mechanisms and consequences of TGF-β-induced EMT during cancer progression. Cell Tissue Res. 2012;347:85–101.View ArticlePubMedGoogle Scholar
- Molina-Montes E, Pérez-Nevot B, Pollán M, Sánchez-Cantalejo E, Espín J, Sánchez MJ. Cumulative risk of second primary contralateral breast cancer in BRCA1/BRCA2 mutation carriers with a first breast cancer: a systematic review and meta-analysis. Breast. 2014;23:721–42.View ArticlePubMedGoogle Scholar
- De Assis S, Hilakivi-Clarke L. Timing of dietary estrogenic exposures and breast cancer risk. Ann N Y Acad Sci. 2006;1089:14–35.View ArticlePubMedGoogle Scholar
- Snyderwine EG. Mammary gland carcinogenesis by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in rats: possible mechanisms. Cancer Lett. 1999;143:211–5.View ArticlePubMedGoogle Scholar
- Hovey RC, McFadden TB, Akers RM. Regulation of mammary gland growth and morphogenesis by the mammary fat pad: a species comparison. J Mammary Gland Biol Neoplasia. 1999;4:53–68.View ArticlePubMedGoogle Scholar
- de Assis S, Khan G, Hilakivi-Clarke L. High birth weight increases mammary tumorigenesis in rats. Int J Cancer. 2006;119:1537–46.View ArticlePubMedGoogle Scholar
- Gouk SW, Cheng SF, Ong AS, Chuah CH. Stearic acids at sn-1, 3 positions of TAG are more efficient at limiting fat deposition than palmitic and oleic acids in C57BL/6 mice. Br J Nutr. 2014;111:1174–80.View ArticlePubMedGoogle Scholar
- Rato L, Alves MG, Cavaco JE, Oliveira PF. High-energy diets: a threat for male fertility? Obes Rev. 2014;15:996–1007.View ArticlePubMedGoogle Scholar
- Chu Y, Huddleston GG, Clancy AN, Harris RB, Bartness TJ. Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior. Endocrinology. 2010;151:5669–79.View ArticlePubMedPubMed CentralGoogle Scholar
- Reame V, Pytlowanciv EZ, Ribeiro DL, Pissolato TF, Taboga SR, Góes RM, Pinto-Fochi ME. Obesogenic environment by excess of dietary fats in different phases of development reduces spermatic efficiency of Wistar rats at adulthood: correlations with metabolic status. Biol Reprod. 2014;91:151.
- Wu H, Hauser R, Krawetz SA, Pilsner JR. Environmental susceptibility of the sperm epigenome during windows of male germ cell development. Curr Environ Health Rep. 2015;2:356–66.View ArticlePubMedPubMed CentralGoogle Scholar
- Gaskins AJ, Colaci DS, Mendiola J, Swan SH, Chavarro JE. Dietary patterns and semen quality in young men. Hum Reprod. 2012;27:2899–907.View ArticlePubMedPubMed CentralGoogle Scholar
- McPherson NO, Fullston T, Aitken RJ, Lane M. Paternal obesity, interventions, and mechanistic pathways to impaired health in offspring. Ann Nutr Metab. 2014;64:231–8.View ArticlePubMedGoogle Scholar
- Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Tyagi S, Holt JE, McLaughlin EA. The microRNA signature of mouse spermatozoa is substantially modified during epididymal maturation. Biol Reprod. 2015;93:91.
- McPherson NP, Fullston T, Bakos HW, Setchell BP, Lane M. Obese father’s metabolic state, adiposity, and reproductive capacity indicate son’s reproductive health. Fertil Steril. 2014;101:865–73.View ArticlePubMedGoogle Scholar
- Fullston T, Ohlsson Teague EM, Palmer NO, DeBlasio MJ, Mitchell M, Corbett M, Print CG, Owens JA, Lane M. Paternal obesity initiates metabolic disturbances in two generations of mice with incomplete penetrance to the F2 generation and alters the transcriptional profile of testis and sperm microRNA content. FASEB J. 2013;27:4226–43.
- Duk MA, Samsonova MG, Samsonov AM. Dynamics of miRNA driven feed-forward loop depends upon miRNA action mechanisms. BMC Genomics. 2014;15 Suppl 12:S9.View ArticlePubMedGoogle Scholar
- Bastea LI, Döppler H, Balogun B, Storz P. Protein kinase D1 maintains the epithelial phenotype by inducing a DNA-bound, inactive SNAI1 transcriptional repressor complex. PLoS One. 2012;7:e30459.View ArticlePubMedPubMed CentralGoogle Scholar
- Ni Y, Wang L, Zhang J, Pang Z, Liu Q, Du J. PKD1 is downregulated in non-small cell lung cancer and mediates the feedback inhibition of mTORC1-S6K1 axis in response to phorbol ester. Int J Biochem Cell Biol. 2015;60:34–42.View ArticlePubMedGoogle Scholar
- Piek E, Moustakas A, Kurisaki A, Heldin CH, ten Dijke P. TGF-β type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells. J Cell Sci. 1999;112:4557–68.PubMedGoogle Scholar
- Guo X, Wang XF. Signaling cross-talk between TGF-β/BMP and other pathways. Cell Res. 2009;19:71–88.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang YE. Non-Smad pathways in TGF-β signaling. Cell Res. 2009;19:128–39.View ArticlePubMedPubMed CentralGoogle Scholar
- Gruber KJ, Haldeman LA. Using the family to combat childhood and adult obesity. Prev Chronic Dis. 2009;6:A106.PubMedPubMed CentralGoogle Scholar
- McPherson NO, Owens JA, Fullston T, Lane M. Preconception diet or exercise intervention in obese fathers normalizes sperm microRNA profile and metabolic syndrome in female offspring. Am J Physiol Endocrinol Metab. 2015;308:E805–21.View ArticlePubMedGoogle Scholar