Volume 3 Supplement 1

23rd Congress of the International Association for Breast Cancer Research

Open Access

Apoptosis-related factors in nonpalpable breast tumors: an immunohistochemical study. Correlation with the mammographic image

  • P Ravazoula1,
  • E Likaki-Karatza2,
  • FA Mpadra2,
  • MV Karamouzis3,
  • P Aroukatos1,
  • E Tzorakoeleptherakis4 and
  • HP Kalofonos3
Breast Cancer Research20013(Suppl 1):A49

https://doi.org/10.1186/bcr376

Received: 10 May 2001

Published: 31 May 2001

Objectives

Retrospective evaluation of the mammographic appearance of nonpalpable breast cancers and correlation with apoptosis-related factors.

Method

Patients with nonpalpable breast lesions (n = 211) were evaluated between 1989 and 1999. All patients underwent preoperative mammographically guided needle-excision biopsy. Specimen radiography was always followed. Histological examination revealed 55 cancers (26%; 30 ductal invasive [54.5%], 18 ductal in situ [32.7%], five lobular invasive [9%] and two lobular in situ [3.8%]). In 41 out of 55 carcinomas, immunohistochemistry was conducted, using monoclonal antibodies against bcl-2, fas and DNA fragmentation, and polyclonal antibody for bax.

Results

Mammography revealed malignant microcalcifications in 42 out of 55 patients (76%) and opacity with undefined borders (greater diameter <1cm) in 13 out of 55 patients (24%). In 16 out of 41 carcinomas (41%) there was immunostain positivity for bcl-2. In seven out of 16 patients (17.5%) mammography showed microcalcifications, whereas opacity was observed in nine out of 16 patients (22.5%). Twenty-three out of 41 carcinomas (56%) were positive for fas. In 14 out of 23 patients (60%) mammography showed microcalcifications and opacity in 11 out of 23 (47%). Thirty out of 41 carcinomas (73%) were positive for bax and DNA fragmentation. In 17 out of 30 (58%) carcinomas that were positive for DNA fragmentation, mammography showed microcalcifica-tions, whereas opacity was revealed in 16 out of 30 (53%) patients. In 18 out of 30 (60%) carcinomas that were positive for bax, mammography showed microcalcifications, whereas opacity was detected in 14 out of 30 (46%) carcinomas.

Conclusion

Our results suggest the existence of significant correlation between mammographic appearance and expression of apoptosis factors in nonpalpable breast cancers, although the number of patients evaluated was relatively small.

Authors’ Affiliations

(1)
Department of Pathology, University Hospital of Patras
(2)
Department of Radiology, University Hospital of Patras
(3)
Division of Oncology 8211; Department of Medicine, University Hospital of Patras
(4)
Department of Surgery, University Hospital of Patras

Copyright

© BioMed Central Ltd 2001

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