Roscovitine confers tumor suppressive effect on therapy-resistant breast tumor cells
© Nair et al.; licensee BioMed Central Ltd. 2011
Received: 29 March 2011
Accepted: 11 August 2011
Published: 11 August 2011
Current clinical strategies for treating hormonal breast cancer involve the use of anti-estrogens that block estrogen receptor (ER)α functions and aromatase inhibitors that decrease local and systemic estrogen production. Both of these strategies improve outcomes for ERα-positive breast cancer patients, however, development of therapy resistance remains a major clinical problem. Divergent molecular pathways have been described for this resistant phenotype and interestingly, the majority of downstream events in these resistance pathways converge upon the modulation of cell cycle regulatory proteins including aberrant activation of cyclin dependent kinase 2 (CDK2). In this study, we examined whether the CDK inhibitor roscovitine confers a tumor suppressive effect on therapy-resistant breast epithelial cells.
Using various in vitro and in vivo assays, we tested the effect of roscovitine on three hormonal therapy-resistant model cells: (a) MCF-7-TamR (acquired tamoxifen resistance model); (b) MCF-7-LTLTca (acquired letrozole resistance model); and (c) MCF-7-HER2 that exhibit tamoxifen resistance (ER-growth factor signaling cross talk model).
Hormonal therapy-resistant cells exhibited aberrant activation of the CDK2 pathway. Roscovitine at a dose of 20 μM significantly inhibited the cell proliferation rate and foci formation potential of all three therapy-resistant cells. The drug treatment substantially increased the proportion of cells in G2/M cell cycle phase with decreased CDK2 activity and promoted low cyclin D1 levels. Interestingly, roscovitine also preferentially down regulated the ERα isoform and ER-coregulators including AIB1 and PELP1. Results from xenograft studies further showed that roscovitine can attenuate growth of therapy-resistant tumors in vivo.
Roscovitine can reduce cell proliferation and survival of hormone therapy-resistant breast cancer cells. Our results support the emerging concept that inhibition of CDK2 activity has the potential to abrogate growth of hormonal therapy-resistant cells.
The steroid hormone estradiol (E2) plays an important role in the initiation and progression of breast cancer. Various biological effects of E2 are mediated through its binding to distinct estrogen receptors (ER), ERα and ERβ, that differ both functionally and structurally [1, 2]. About 70% of breast cancer patients are ERα positive at the time of presentation . Upon E2 binding, ERα mediates regulation of target gene transcription and cell cycle progression via recruitment of co-regulators . Emerging evidence suggests that ERβ decreases cell proliferation, that breast tumors express low levels of ERβ and that the ratio between ERα and ERβ is the driving force for tumor cell proliferation . Current endocrine therapy for ER-positive breast cancer involves modulating the ER-pathway using either anti-estrogens (AEs) or aromatase inhibitors (AIs). Despite the positive effects, de novo and/or acquired resistance to endocrine therapies frequently occur . Although mechanisms for hormonal therapy resistance remains elusive, emerging data suggest that ER cross talk with human epidermal growth factor receptor 2 (HER2)-, Src-, and AKT-pathways, that alterations in the levels of ER subtypes, and that deregulation of co-regulator are major causes of resistance [6–8].
Interestingly, most downstream events in these resistance signaling pathways converge upon modulation of cell cycle regulatory proteins; the most conspicuous of which is the upregulation of cyclins E and A, along with activation of cyclin dependent kinase 2 (CDK2) [9, 10]. The cell cycle machinery is also a prime target for both estrogen and AEs to enhance cell cycle progression or to induce cell cycle arrest, respectively . CDK2 is known to aid in cancer cell proliferation by modulating E2F-pRB pathway  and is also shown to enhance ligand-independent activation of ERα [13, 14]. The expression of RB/E2F target genes, which is tightly controlled by CDK2 activity, is often deregulated and associated with worse prognosis for tamoxifen-treated breast cancer patients . Collectively, these emerging studies strongly support the concept that CDK2 activity is a critical component for the generation of a hormone therapy-resistant phenotype and that blocking of CDK2 activity may be useful as a therapeutic strategy for therapy-resistant patients.
(R)-stereoisomer of roscovitine (Seliciclib or CYC202) is one of the extensively studied CDK inhibitors, both in vitro and in vivo . Roscovitine is the first, selective, orally bioavailable inhibitor of CDKs to enter clinical trials  and is currently in phase II trials for B-cell malignancies, and lung cancer . It predominantly inhibits CDK2, and has a very short half-life with no known active metabolites . Earlier studies have shown that roscovitine promotes accumulation of breast tumor cells in G2/M phase [20, 21], potentiates the anti-tumor effects of doxorubicin on breast cancer cells , and has a synergistic antitumor effect with irradiation in a breast cancer xenograft model . Although these studies suggested that roscovitine may have therapeutic utility in the management of hormonal therapy-sensitive breast cancer cells, the utility of roscovitine to suppress endocrine therapy-resistant breast cancer cells has not been explored.
In this study, we evaluated the tumor-suppressive effect of roscovitine using three different breast cancer models that exhibit resistance to hormonal therapy. Our findings using in vitro and in vivo xenograft assays demonstrate that roscovitine has the potential to reduce growth of all three therapy-resistant cells. Mechanistic studies revealed that roscovitine actions involve both blocking of CDK functions as well as down-regulation of ERα. As roscovitine is currently in clinical trials, our findings may have a high translational potential, suggesting that roscovitine represents a novel therapeutic drug for treating therapy-resistant tumors.
Materials and methods
Model cells and reagents
MCF7 cells were purchased from American-type culture collection (ATCC, Manassas, VA, USA), endocrine-resistant model cells MCF7-HER2 , MCF7-tamoxifen resistant (TamR)  and long-term letrozole treated MCF7ca (MCF7LTLTca)  were described. MCF7-LTLTca and MCF7-TamR cells were cultured in phenol red free-RPMI medium containing 5% dextran charcoal-treated serum with either 1 μmol/L of letrozole or 1 μmol/L tamoxifen, respectively. The roscovitine that was used for the in vitro studies was purchased from Calbiochem (San Diego, CA, USA) and that was used for the in vivo studies was purchased from LC Laboratories (Woburn, MA, USA). Antibodies for phospho-Rb, phospho-CDK2, and AIB1 were purchased from Cell Signaling (Beverly, MA, USA). The antibody for PELP1 was purchased from Bethyl Laboratories (Montgomery, TX, USA). ERα was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and Thermo Fisher Scientific (Rockford, IL, USA). terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) kit for apoptosis detection was purchased from (Roche, Mannheim, Germany) and Proliferating Cell Nuclear Antigen (PCNA) antibody was purchased from Vector Lab (Burlingame, CA, USA).
Cell lysis and western blot analysis
Cells were washed with ice cold 1 × PBS and lysis was conducted using a modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, 50 mM NaF, 5 mM EDTA, 0.5% (wt/vol) sodium deoxycholate and 1% Triton X-100) containing phosphatase and protease inhibitors. Lysates were run on either 7% or 8% SDS-PAGE. Western blot analysis was performed with phospho- and total antibodies. For protein degradation analysis, model cells were pretreated with MG132 (5 μM) for one hour prior to treatment with 20 μM roscovitine.
Cell proliferation assay
The cell proliferation rate was measured in a 96-well, flat, clear-bottom, opaque-wall microplates using Cell Titer-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA). Model cells (2 × 103) were plated in each well and cultured for 24 hours before treatment with or without various doses of roscovitine for another 72 hours. Total ATP content as an estimate of total number of viable cells was measured by luminescence-based assay using automatic Fluoroskan Luminometer (Thermo Scientific Waltham, MA, USA). Roscovitine was dissolved in dimethyl sulfoxide (DMSO) as 2.8 mM stock solution and stored as aliquots at -20°C. All roscovitine treatments were performed in phenol red-free medium as described .
Model cells were plated in six-well plates at a density of 1 × 103 cells per well in triplicate and treated with or without 10 μM roscovitine for seven days. After three weeks, cells were fixed in cold-methanol, and stained with 0.5% crystal violet. Image acquisition was conducted using a digital camera.
Model cells were plated in 100 mm plates and treated with or without 20 μM roscovitine for 24 hours. Cells were trypsinized and harvested in 1 × PBS, followed by fixation in ice cold 70% ethanol. Staining was done with a mixture of 50 μg/mL propidium iodide and 50 μg/mL RNase A. Cell cycle status was quantified by using a FACS-Calibur flow cytometer.
Quantitative RT-PCR analysis
Model cells were plated in 100 mm plates and treated with or without roscovitine (20 to 30 μM) for 24 hours. Cells were harvested with Trizol Reagent (Invitrogen, Carlsbad, CA, USA) and total RNA was isolated according to the manufacturer's instructions. cDNA synthesis was done using Superscript III RT-PCR kit (Invitrogen, Carlsbad, CA, USA). Real-time PCR was done using a Cepheid Smartcycler II (Sunnyvale, CA, USA) with specific real-time PCR primers for the ERα (ESR1-F: 5'AGAGGGAAAGTAGGGCAGAA 3' and ESR1-R: 5'TGGGAAATGAAGAAGAGCTG 3'). Results were normalized to actin transcript levels and the difference in fold expression was calculated using delta-delta-CT method.
In vivoxenograft assays
All animal experiments were performed after obtaining University of Texas Health Sciences Centre at San Antonio (UTHSCSA) institutional animal care committee (IACUC) approval and the animals were housed in accordance with UTHSCSA institution's protocol for animal experiments. Model cells (5 × 106) cells mixed with an equal volume of Matrigel were implanted subcutaneously into the flanks of six to seven-week-old female nude mice as described . For MCF7-HER2 xenografts, nude mice were also subcutaneously implanted with estrogen pellets (Innovative Research, Sarasota, FL, USA) ; whereas nude mice with MCF7-TamR and MCF7-LTLTca xenografts were given subcutaneous injection of tamoxifen (100 μg/day/mice) or Δ4-androstenedione (100 μg/day/mice), respectively, as described . Roscovitine treatment was initiated after three weeks of inoculation when tumors reached measurable size. Roscovitine suspension was prepared in 50 mM HCl solution as described earlier  and administered orally at a dose of 100 mg/kg body weight (three times a day) for 10 consecutive days (total 30 doses). Tumor volumes were measured with a vernier caliper at weekly intervals. After the 25th day, the mice were euthanized, and the tumors were removed, weighed and processed for immunohistochemistry (IHC) staining. Tumor volume calculation was performed using modified ellipsoidal formula: tumor volume = 1/2(L × W2), where L is the longitudinal diameter and W is the transverse diameter . Body weight was measured at weekly intervals to rule out the drug toxicity. Student t-test was used to assess the statistical difference between control and roscovitine-treated groups. The level of significance was set at P < 0.05.
Immunohistochemistry and TUNEL assay
IHC analysis was performed as described . The dilution of the PCNA antibody used for IHC was 1 in 100. After washing with 1 × PBST, slides were further incubated in DAKO universal secondary LINK solution for 30 minutes and horseradish peroxidase-labeled streptavidin for 20 minutes at room temperature. Successful staining was visualized using the DAB substrate (Vector Lab, Burlingame, CA, USA), followed by counterstaining using Mayer's hematoxylin (Vector Lab, Burlingame, CA, USA). TUNEL staining was performed following manufacturer's instructions (Roche, Indianpolis, IN).
Roscovitine suppresses proliferation and survival of therapy-resistant cells
Roscovitine arrests the cell cycle in endocrine-resistant cells
Roscovitine down regulates key cell cycle regulators and ERα levels
Therapeutic efficacy of roscovitine on xenografts generated from endocrine-resistant model cells
Breast cancer is the most common cancer among women in the USA and patients with ERα-positive tumors greatly benefit from existing hormonal therapies using AEs and AIs. [33–36]. However, many patients exhibit de novo or acquired resistance to hormonal therapies. This resistance represents a major clinical problem. Emerging findings suggest that deregulation of cell cycle components such as CDK2 axis has the potential to contribute both to cell cycle progression and to endocrine resistance . In this study, we explored the hypothesis that targeting the CDK2 axis using roscovitine will have therapeutic benefit. Using three model cells that exhibit endocrine therapy resistance, we found that (1) therapy-resistant cells exhibit greater CDK2 activation; (2) roscovitine treatment decreased CDK2 activity and promoted G2/M or G1 accumulation of resistant cells; (3) roscovitine treatment significantly reduced proliferation of the resistant cells; (4) roscovitine preferentially down regulated the ERα isoform and its associated signaling components; and (5) preclinical xenograft-based studies found roscovitine had a therapeutic efficacy by attenuating the growth of resistant tumors. Collectively, these results suggest that CDK2 signaling confers a growth advantage to resistant cells and that roscovitine treatment represents a feasible strategy for therapeutic targeting of hormonal therapy resistance.
Tumor cells exhibit oncogenic addiction. Recent studies suggest that interphase CDKs (CDK4, CDK2) are only essential for proliferation of tumor cells and selective CDK inhibition may provide therapeutic benefit . Previous studies demonstrated the potential of roscovitine to abrogate cell proliferation and to induce cell cycle arrest in both ER-positive cells [21, 26, 38] and ER-negative cells . In this study, we demonstrated that roscovitine exhibited a profound growth inhibitory effect on hormone-resistant model cells. Roscovitine treatment promoted G2/M arrest in MCF7-TamR and MCF7-HER2 cells and arrested in LTLTca cells in the G1 phase. These findings are in agreement with the findings from published studies that concluded roscovitine has the potential to arrest cell cycle predominantly at G2/M phase and occasionally in the G1 phase [22, 38, 40, 41]. The differential effect of roscovitine on cell cycle status in LTLTca compared with TamR and HER2 cells is currently unknown, but we speculate the difference may lie in differential activation of cell cycle checkpoints in these model cells. Utility of roscovitine against letrozole-resistant breast cancer cells have recently been demonstrated  and our data strongly corroborate this study.
Activation of the CDK2 axis is considered a vital end point of various molecular pathways leading to hormone-therapy resistance . Cyclin E, an activator of CDK2, when ectopically over-expressed is able to abrogate the anti-proliferative actions of tamoxifen on breast cancer cells  and is also shown to be a good indicator for endocrine-therapy failure [43, 44]. Recent studies showed that cyclin E can undergo proteolytic cleavage to create low molecular weight (LMW) cyclin E variants  and these LMW cyclin E variants lacking the amino-terminus could significantly augment the tamoxifen therapy resistance by activating CDK2 functions . LMW cyclin E is also reported to abrogate the anti-proliferative activity of the AI letrozole . Overexpression of cyclin A in breast cancer also significantly correlates with poor outcome in tamoxifen-treated patients . Down regulation of CDK inhibitors (p21 and p27) have been implicated in the development of hormone therapy resistance [48, 49]. In this study, we show that roscovitine, a potent inhibitor of CDK2, can curb the growth of therapy-resistant breast cancer cells and to down regulate expression of ERα. As deregulation of the cell cycle machinery and ER signaling both contribute to hormonal therapy resistance, a roscovitine treatment regime that suppresses both these pathways could serve as a double-edged sword to interfere with the hormonal therapy-resistant mechanisms.
Cell cycle regulators cyclins and CDKs are the key proteins that aid in cell proliferation by modulating the E2F-RB pathway . Earlier studies that showed functional ablation of retinoblastoma protein (pRb) leads to activation of CDK2 in breast cancer [15, 50] and concordantly, that deregulation of E2F transcription factor target genes associated with poor prognosis . Our results suggested that roscovitine can reduce phosphorylation of Thr160 in CDK2 (a marker of CDK2 activation), reduce phosphorylation of pRb (a well-known substrate of CDK2) at Ser795, and reduce levels of cyclin D1 in therapy-resistant cells. The ability of roscovitine to reduce pRb phosphorylation and to alter the status of cyclin D1, suggest that roscovitine therapy may have therapeutic benefit on clinical cases with CDK2 activation and deregulation of E2F functions.
Cross talk between the cell cycle machinery and ER pathways has been well documented in the context of endocrine resistance . CDKs are known to potentiate ER functions in AE-resistant cells  and CDK2 activity significantly correlates with poorer five-year relapse-free survival in patients . Interestingly, in our study, in vitro roscovitine treatment reduced the expression of both ERα and ER co-regulators such as AIB1 and PELP1, which are commonly implicated in therapy resistance [31, 32]. Our findings suggest that the reduction in ERα levels in the resistant cells is due to both transcriptional and post-translational effects by roscovitine. Our data corroborate data from a recent study that demonstrated a reduction in ERα levels in MCF7 cells after roscovitine treatment, which possibly occurred through the inhibition of CDK7 . As many endocrine therapy-resistant cells retain expression and functionality of ERα, the ability of roscovitine to down regulate the ERα axis, may also have contributed to its ability to curb the progression of the resistant cells.
(R)-roscovitine is currently in early clinical trials to examine its effects on treating non-small cell lung cancer and advanced solid tumors . The oral bioavailability of (R)-roscovitine is a great advantage for its possible future clinical use. In this study, we tested efficacy of roscovitine in vivo by using a xenograft transplantation assay. Our data suggest that roscovitine has strong tumor suppressive effects on endocrine-resistant xenograft tumors. To avoid possible side effects of roscovitine, we chose a rather moderate dose (100 mg/kg) of the drug; a few other studies used 400 mg/kg roscovitine . IHC analysis of tumors revealed a significant decrease in proliferation along with an increase in apoptosis. Our data corroborate data from other studies that demonstrated the apoptotic potential of roscovitine [39, 54] and in turn suggest that multiple pathways are responsible for roscovitine-induced tumor suppressive effects.
Our results support the concept that inhibition of CDK2 activity has the potential to abrogate growth of hormonal therapy-resistant cells. Further, the results from this study provide strong in vitro and in vivo evidence that roscovitine confers a tumor-suppressive effect on endocrine therapy-resistant breast tumor cells by inhibiting CDK functions and altering expression of ERα and ER-coregulators, and by promoting apoptosis. Future studies are needed to examine whether roscovitine treatment can be used to treat advanced and therapy-resistant breast cancer and whether roscovitine treatment can complement the existing therapies.
cyclin dependent kinase
human epidermal growth factor receptor 2
low molecular weight
long-term letrozole treated MCF7ca
Proliferating Cell Nuclear Antigen
terminal deoxynucleotidyl transferase dUTP nick end labeling.
This study is supported by NIH grant CA0095681 (R.K. Vadlamudi) and Department of Defense pre-doctoral grant W81XWH-09-1-0010 (B.C. Nair).
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