Estrogen receptor-beta sensitizes breast cancer cells to the anti-estrogenic actions of endoxifen
© Wu et al.; licensee BioMed Central Ltd. 2011
Received: 18 August 2010
Accepted: 10 March 2011
Published: 10 March 2011
We have previously demonstrated that endoxifen is the most important tamoxifen metabolite responsible for eliciting the anti-estrogenic effects of this drug in breast cancer cells expressing estrogen receptor-alpha (ERα). However, the relevance of ERβ in mediating endoxifen action has yet to be explored. Here, we characterize the molecular actions of endoxifen in breast cancer cells expressing ERβ and examine its effectiveness as an anti-estrogenic agent in these cell lines.
MCF7, Hs578T and U2OS cells were stably transfected with full-length ERβ. ERβ protein stability, dimer formation with ERα and expression of known ER target genes were characterized following endoxifen exposure. The ability of various endoxifen concentrations to block estrogen-induced proliferation of MCF7 parental and ERβ-expressing cells was determined. The global gene expression profiles of these two cell lines was monitored following estrogen and endoxifen exposure and biological pathway analysis of these data sets was conducted to identify altered cellular processes.
Our data demonstrate that endoxifen stabilizes ERβ protein, unlike its targeted degradation of ERα, and induces ERα/ERβ heterodimerization in a concentration dependent manner. Endoxifen is also shown to be a more potent inhibitor of estrogen target genes when ERβ is expressed. Additionally, low concentrations of endoxifen observed in tamoxifen treated patients with deficient CYP2D6 activity (20 to 40 nM) markedly inhibit estrogen-induced cell proliferation rates in the presence of ERβ, whereas much higher endoxifen concentrations are needed when ERβ is absent. Microarray analyses reveal substantial differences in the global gene expression profiles induced by endoxifen at low concentrations (40 nM) when comparing MCF7 cells which express ERβ to those that do not. These profiles implicate pathways related to cell proliferation and apoptosis in mediating endoxifen effectiveness at these lower concentrations.
Taken together, these data demonstrate that the presence of ERβ enhances the sensitivity of breast cancer cells to the anti-estrogenic effects of endoxifen likely through the molecular actions of ERα/β heterodimers. These findings underscore the need to further elucidate the role of ERβ in the biology and treatment of breast cancer and suggest that the importance of pharmacologic variation in endoxifen concentrations may differ according to ERβ expression.
Each year, nearly 1.3 million women are diagnosed with breast cancer worldwide and about two-thirds of these individuals are determined to have hormone sensitive tumors based on the expression of estrogen receptor-alpha (ERα). Tamoxifen, a selective estrogen receptor modulator (SERM), remains an important therapeutic agent in the treatment of women with endocrine sensitive breast cancer as it is known to effectively inhibit the proliferation-inducing effects of 17β-estradiol (estrogen) in ERα positive breast tumor cells.
Like many drugs, tamoxifen is extensively metabolized in the body by the cytochrome P450 enzyme system resulting in the production of three primary metabolites; 4-hydroxytamoxifen (4HT), N-desmethyl-tamoxifen (NDT) and endoxifen [1–3]. Recent reports have demonstrated that steady state circulating levels of tamoxifen, 4HT, and NDT in women receiving the standard dose of tamoxifen therapy (20 mg/day) are 300 nM, 7 nM, and 700 nM, respectively . However, plasma endoxifen concentrations are highly variable, ranging from 5 to 180 nM, due to the activity of the cytochrome P450 2D6 (CYP2D6) mediated oxidation of NDT . Prospective studies have demonstrated that genetic CYP2D6 polymorphisms, and drugs, which reduce or abrogate CYP2D6 enzyme activity, significantly decrease endoxifen plasma concentrations [3–5]. These findings encouraged investigators to examine the hypothesis that CYP2D6 genotype status, and thus endoxifen concentrations, would affect clinical outcome in women treated with tamoxifen for their breast cancer. Although some controversy remains, the majority of the reports indicate a relationship between CYP2D6-related low levels of endoxifen and poor outcomes [6–15]. Past studies from this laboratory support these clinical findings as we have demonstrated that endoxifen is the most potent tamoxifen metabolite responsible for inhibiting estrogen induced gene expression changes and proliferation rates in ERα positive breast cancer cells at clinically relevant concentrations . At this time, the clinical development of endoxifen is ongoing, with NCI supported phase I studies of endoxifen hydrochloride set to commence in early 2011 at both the Mayo Clinic and NCI.
Tamoxifen and its metabolites are known to function by blocking the effects of estrogen, a steroid hormone that binds to, and activates, two main ER isoforms, ERα and ERβ. The role of ERα in breast cancer has been studied extensively for years, and its protein expression remains the most important biomarker in the treatment of this disease. However, the potential functions of ERβ in the progression and treatment of breast cancer have largely remained a mystery. In vitro studies have revealed that the actions of these two receptors are drastically different at the level of gene expression, both in response to estrogen and anti-estrogens [17–23]. Numerous reports have demonstrated that exposure of ERα expressing breast cancer cells to estrogen results in increased rates of proliferation while more recent studies have suggested that expression of ERβ alone [21, 24, 25], or in combination with ERα [26–28], inhibits cell proliferation following estrogen exposure.
The summation of these in vitro studies suggests that ERβ may function as a tumor suppressor. A number of clinical studies have revealed that the presence of ERβ protein in breast tumors correlates with improved rates of recurrence, disease-free survival and overall survival [29–38] while others indicate little correlation [39–41] or even worse prognosis [42, 43]. Additional studies have suggested that the expression of ERβ in breast tumors increases the effectiveness of tamoxifen therapy [44–46] and one report found that 47% of breast tumors classified as ERα negative express ERβ . These observations highlight the need to further define the relevance of ERβ in breast cancer progression and treatment.
Based on the foregoing, the objective of the present study was to determine the role of ERβ in mediating endoxifen action in breast cancer cells. The results of this study demonstrate that ERβ enhances the anti-estrogenic effects of endoxifen in breast cancer cells likely through the actions of ERα/β heterodimers, and suggest that the achievement of higher endoxifen concentration may not be necessary in patients whose tumors express ERβ and that these same patients may benefit from tamoxifen therapy regardless of their CYP2D6 genotype.
Materials and methods
Cell culture, chemicals and reagents
MCF7 cells were generously provided by Dr. Robert Clarke (Georgetown University) and were cultured in phenol red-free Dulbecco's modified Eagle's medium/F12 (DMEM/F12) medium containing 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (AA) solution in a humidified 37°C incubator with 5% CO2. MCF7 cells stably expressing ERβ were generated using an S-tagged-Flag-tagged ERβ expression construct (pIRES2-EGFP) developed in our laboratory. Individual MCF7-ERβ clones were isolated following selection with 300 μL/mL G418. Doxycycline inducible Hs578T-ERβ, U2OS-ERβ and U2OS-ERα/β cells lines were cultured as previously described [19–21]. All cell treatments were conducted in phenol red-free DMEM/F12 medium containing 10% triple charcoal stripped FBS. 17β-estradiol and doxycyline were purchased from Sigma Aldrich (St. Louis, MO, USA). PPT (propyl pyrazole triol) and DPN (diarylpropionitrile) were purchased from Tocris Biosciences Inc. (Baldwin, MO, USA). (Z)-endoxifen was synthesized by Dr. Abdul Fauq (Mayo Clinic, Jacksonville, FL, USA).
The polyclonal ERβ specific antibody utilized in this study was developed by this laboratory. Briefly, a protein fragment of ERβ spanning amino acids 1 to 140 was cloned into the pGEX-5X-3 vector (Life Technologies, Carlsbad, CA, USA) and expressed in DH5α bacterial cells. Purified ERβ protein was immunized in rabbits by Cocalico Biologicals Inc (Reamstown, PA, USA). ERβ specific antibodies were purified from serum using an affinity purification column containing the ERβ protein fragment. We have extensively characterized this antibody through Western blotting, immunoprecipitation, immunohistochemistry and immunoflourescence using multiple cell model systems which express either no ERs, ERα alone, ERβ alone or a combination of ERα and ERβ, to ensure its specificity for the β isoform. These data have revealed that this antibody is highly sensitive for the detection of ERβ and exhibits no cross-reaction with ERα or other proteins (data not shown). ERα (H-20) antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Flag (M2) and α-Tubulin (DM 1A) antibodies were purchased from Sigma Aldrich.
All cell lysates were harvested using NETN buffer (150 mM NaCl, 1 mM EDTA, 20 mM Tris (pH 8.0), 0.5% Nonidet P-40) and insoluble material was pelleted. Protein concentrations were determined using Bradford Reagent and equal amounts of cell lysate were separated by SDS-PAGE. Proteins were transferred to PVDF membranes, probed with primary and secondary antibodies and visualized using enhanced chemiluminescence (Amersham Biosciences, Piscataway, NJ, USA).
Immunofluorescent staining analysis
MCF7-ERβ cells were plated on cover slides at approximately 50% confluence and fixed with cold methanol for one hour followed by permeabilization with 0.1% Triton X-100 for five minutes on ice. Slides were pre-incubated in 5% goat serum for one hour followed by exposure to Flag antibody for another hour. Slides were washed three times with 1× PBS and subsequently incubated with Rhodamine-labeled anti-mouse secondary antibody (Sigma-Aldrich) for 30 minutes at room temperature. Nuclei were simultaneously stained with DAPI (Sigma-Aldrich). Immunofluorescent detection was conducted using a Zeiss Laser Scanning Microscope 510 (Carl Zeiss, Jena, Germany)
MCF7-ERβ cells were plated in 12-well tissue culture plates at approximately 70% confluence and subsequently transfected in triplicate with 250 ng per well of the ERE-TK-luciferase reporter construct using Fugene 6 (Roche Applied Science, Indianapolis, IN, USA). Following transfection, cells were treated as indicated for 24 h. Cells were lysed in 1× Passive Lysis Buffer (Promega, Madison, WI, USA) and equal amounts of extract were assayed for luciferase activity.
MCF7-ERβ or U2OS-ERα/β cells were plated at a density of approximately 50% in 100 mm tissue culture plates. U2OS cells were treated with doxycycline as previously described to induce expression of ERα and ERβ  and subsequently exposed to indicated concentrations of endoxifen. Following 24 hours of incubation, cells were washed twice with PBS and lysed in NETN buffer. Equal amounts of cell lysates were immunoprecipitated at 4°C overnight using 1 μg of either Flag or ERβ antibody. Protein complexes were purified using protein G beads, separated by SDS-PAGE, transferred to PVDF membranes and blocked in 5% milk overnight. Western blotting was performed using indicated antibodies as described above.
Real-time reverse transcriptase polymerase chain reaction
MCF7 and MCF7-ERβ cells were plated in 12-well tissue-culture plates and treated in triplicate as indicated for 24 hours. Total RNA was isolated using Trizol reagent (Invitrogen) and 500 ng was reverse transcribed using the iScript™ cDNA Synthesis Kit (Bio-Rad). Real-time PCR was performed in triplicate using a Bio-Rad iCycler (Hercules, CA, USA) and a PerfeCTa™ SYBR Green Fast Mix™ for iQ real-time PCR kit (Quanta Biosciences, Gaithersburg, MD, USA) as specified by the manufacturer. Quantitation of the PCR results were calculated based on the threshold cycle (Ct) and were normalized using TATA Binding Protein as a control. All PCR primers were designed using Primer3 software  and were purchased from Integrated DNA Technologies (Coralville, IA, USA). Primer sequences are provided in Additional file 1.
Cell proliferation assays
MCF7 and MCF7-ERβ cells were grown in 10% triple charcoal-stripped serum-containing medium for three days and subsequently plated at a density of 2,000 cells per well in 96-well tissue culture plates. Cells were treated with vehicle, 1 nM estrogen or 1 nM estrogen plus increasing concentrations of endoxifen (20 to 1,000 nM) for eight days. Culture medium and treatments were replaced every other day. Proliferation rates were determined using a CellTiter-Glo Luminescent Cell Viability kit (Promega).
Illumina microarray analysis
Changes in the gene expression profiles of MCF7 and MCF7-ERβ cells elicited by either 1 nM estrogen alone or estrogen plus 40 nM endoxifen were determined using the Illumina HumanHT-12 expression BeadChip platform to screen more than 27,000 annotated genes represented by 48,804 probes by Mayo Clinic's Advanced Genomics Technology Center (Rochester, MN, USA). Data were processed using BeadStudio Version 3.1 and normalized using the fastlo function  implemented in the statistical package R. Data were filtered to exclude probes (referred to as genes throughout) whose expression was at or below background levels as determined by detection P-values (≥0.05). Pair-wise comparisons were made to identify differentially expressed genes using Linear Models for Microarray Data (LIMMA). Genes were determined to be significantly regulated if their differential P-value was < 0.05 between groups. Fold changes were calculated by raising 2 to the power of mean difference (log 2 scale) between the treatment groups and controls. The normalized and raw microarray data presented here are available in the Gene Expression Omnibus  under the accession number: [GEO:GSE27375].
Biological pathway analysis
Genes determined to be significantly regulated by the addition of 40 nM endoxifen relative to estrogen alone in both parental and ERβ expressing MCF7 cells were further analyzed using MetaCore software to identify differences in biological pathways altered between the two cell lines. Genes with differential expression P-values < 0.05 from each comparison were used as focus genes and a hypergeometric test was applied to each of over 600 canonical pathways. Enriched pathways with P-values < 0.05 were suggested to be significantly regulated by the addition of 40 nM endoxifen within each cell line. Adjustment for multiple comparisons was conducted using a false discovery rate of 0.25.
Development and characterization of MCF7-ERβ cell lines
ERβ protein levels are stabilized by endoxifen
Since we have previously demonstrated that endoxifen exposure results in rapid turnover of ERα protein in multiple cell types through proteasomal degradation , it was of interest to determine the effects of endoxifen on ERβ protein levels. Unlike that of ERα, endoxifen exposure resulted in stabilization of ERβ protein in MCF7-ERβ cells in a concentration dependent manner (Figure 2A). These results were confirmed in Hs578T breast cancer cells and U2OS osteosarcoma cells stably expressing ERβ (Figure 2B, C).
Endoxifen induces ERα/β heterodimer formation
Given that ERβ protein levels are stabilized by endoxifen and that ERβ interacts with ERα, we next sought to determine if endoxifen exposure resulted in ERα/β heterodimer formation. Immunoprecipitation assays demonstrate that endoxifen induces ERα/β heterodimer formation in MCF7-ERβ cells which results in the stabilization and accumulation of ERα protein levels (Figure 3A). These studies were conducted in U2OS cells stably expressing both ER isoforms and similar results were observed (Figure 3B). The results of these studies demonstrate that exposure of cells which express both ER isoforms to endoxifen results in stabilization and accumulation of both ERα and ERβ protein likely due to its induction of heterodimer formation. It is speculated that ERα and ERβ homodimer formation likely occurs to some degree as well.
Endoxifen's ability to inhibit estrogen induced gene expression and proliferation is enhanced by ERβ
Our laboratory previously characterized the inhibition of estrogen induced gene expression and proliferation by endoxifen in MCF7 cells . In order to determine the effects of ERβ expression on the anti-estrogenic actions of endoxifen, we next compared the ability of endoxifen to inhibit estrogen induction of known ERα target genes in parental and ERβ expressing MCF7 cells. The expression levels of cyclinD1, PS2, progesterone receptor and amphiregulin were monitored in both cell lines by real-time PCR following treatment with estrogen alone or estrogen plus increasing concentrations of endoxifen. In contrast to the ERE data presented in Figure 1, estrogen treatment further stimulated the expression of three of the four genes (PS2, PGR and AREG) in ERβ expressing cells relative to the parental cell line (Figure 4A-D). Interestingly, low concentrations of endoxifen (20 nM) significantly inhibited the estrogen induction of three of the four genes (CCND1, PS2 and PGR) only in ERβ expressing cells while higher endoxifen concentrations (100 to 1,000 nM) resulted in similar patterns of gene expression between the two cell lines (Figure 4A-D). These data suggest that expression of ERβ, in ERα positive breast cancer cells, enhances the anti-estrogenic properties of endoxifen.
ERβ expression results in unique gene expression patterns following estrogen and endoxifen exposure
We next compared the microarray results of parental and MCF7-ERβ expressing cells treated with estrogen plus 40 nM endoxifen to estrogen treatment alone. Using the same selection criteria as above, significantly fewer genes were determined to be regulated by this dose of endoxifen. In the parental cell line, the expression levels of 75 genes were altered by at least 1.5-fold by the addition of endoxifen of which 44 were increased and 31 were decreased (Figure 6C). Similar results were observed in the MCF7-ERβ cell line in that a total of 78 genes were differentially regulated in response to endoxifen with 37 exhibiting increased expression and 41 exhibiting decreased expression (Figure 6C). Comparison of these two data sets indicated that 46 genes (43%) were commonly regulated in both cell lines while 29 (27%) were unique to the parental cells and 32 (30%) were unique to the ERβ expressing cells (Figure 6C). A list of these genes and their detected fold changes is provided in Additional file 4. As above, two genes exhibiting increased expression and two genes exhibiting decreased expression in response to endoxifen treatment were randomly selected for each cell line and confirmed by real-time PCR. The relative expression levels of these genes as determined by both microarray and real-time PCR following estrogen treatment alone and estrogen plus endoxifen are shown in Figure 6D.
Pathway analysis identifies specific biological processes that are uniquely regulated by endoxifen in MCF7-ERβ cells
Biological pathways regulated by estrogen plus 40 nM endoxifen relative to estrogen treatment alone
MCF7 Parental Cells
Immune response_Antiviral actions of Interferons
Immune response_IFN gamma signaling pathway
Regulation of lipid metabolism_Regulation of fatty acid synthase activity
Neurodisease_Parkin disorder under Parkinson disease
Regulation of lipid metabolism_Regulation of acetyl-CoA carboxylase 1
Cytoskeleton remodeling_Thyroliberin in cytoskeleton remodeling
CFTR-dependent regulation of ion channels in Airway Epithelium
Immune response_IL-27 signaling pathway
Development_A1 receptor signaling
Neurophysiological process_PGE2-induced pain processing
Immune response_Th1 and Th2 cell differentiation
wtCFTR and deltaF508 traffic/Membrane expression (norm and CF)
Regulation of lipid metabolism_Regulation of fatty acid synthase activity
Immune response_IL-27 signaling pathway
Blood coagulation_Blood coagulation
Cell cycle_ERα regulation of G1/S transition
Globo-(isoglobo) series GSL Metabolism
Neurodisease_Parkin disorder under Parkinson disease
ERα action on cytoskeleton remodeling and cell migration
Transcription_Ligand-Dependent Transcription of Retinoid-Target genes
ENaC regulation in airways (normal and CF)
CFTR-dependent regulation of ion channels in Airway Epithelium
wtCFTR and delta508-CFTR traffic/Generic schema (norm and CF)
Endocrine sensitive breast cancer
The large majority of breast cancer patients display tumors that are estrogen dependent based on the expression of ERα. Deprivation of estrogen signaling, most commonly through the use of tamoxifen in the adjuvant setting, typically results in tumor regression. However, the use of ERα alone as an indicator of responsiveness to anti-estrogens is far from perfect as about one-half of ERα positive tumors do not respond to tamoxifen therapy and about 10% of ERα negative tumors do respond. These studies demonstrate that other estrogen and anti-estrogen receptors and/or signaling pathways may be involved in mediating the responsiveness of endocrine sensitive tumors to hormonal agents. Following the discovery of ERβ, investigators have sought to uncover the role that this protein may play in mediating breast cancer progression and treatment. Here, we demonstrate that expression of ERβ in ERα positive MCF7 cells significantly enhances the anti-estrogenic effects of endoxifen. This study provides evidence that endoxifen stabilizes ERβ protein levels and induces ERα/β heterodimer formation which results in differential gene expression patterns. Perhaps most importantly, our studies reveal that even low concentrations of endoxifen, mimicking that of poor and intermediate CYP2D6 metabolizers, results in repression of estrogen induced breast cancer proliferation in cells expressing ERβ, but not in those that express only ERα.
Role of ERβ in breast cancer
ERβ is known to be expressed in normal breast epithelial cells and several studies have demonstrated that ERβ expression levels are suppressed in many breast cancers [51–54]. However, re-expression of ERβ in ER negative breast cancer cells has been shown to reduce both basal and estrogen induced proliferation rates [21, 24, 25]. Expression of ERβ in ERα positive breast cancer cells also results in suppression of proliferation following estrogen exposure [26–28]. Furthermore, ERβ expression has been shown to increase the effectiveness of high concentrations of SERMs such as 4HT, raloxifen and fulvestrant [28, 55]in vitro. While the latter studies did not analyze endoxifen, nor did they utilize clinically relevant concentrations of 4HT (plasma concentrations are less than 10 nM in patients receiving 20 mg/day), they do further implicate a role for ERβ in mediating anti-estrogenic activities.
It is possible that the increased effectiveness of endoxifen in ERβ expressing MCF7 cells is related to the molecular actions of ERα/β heterodimers since we demonstrate that heterodimer formation is induced in a concentration dependent manner following endoxifen exposure. Indeed, the global gene expression changes induced by estrogen and anti-estrogens are known to be different in cells expressing both estrogen receptors relative to cells expressing only ERα or ERβ [20, 21, 56]. The results of the present study also demonstrate that both estrogen and endoxifen regulate unique subsets of genes in MCF7 cells expressing both receptors relative to cells expressing ERα alone. Biological pathway analysis of endoxifen regulated genes revealed that the majority of altered pathways are unique to either the parental or ERβ expressing cell lines. Pathways involving ERα mediated cell cycle regulation and cell migration were only affected in the presence of ERβ suggesting that the increased effectiveness of endoxifen may be through the inhibition of ERα activity by ERβ.
Compounds which induce ER heterodimer formation as therapeutic drugs
The ability of specific compounds to induce ER heterodimer formation is of significant importance since these two receptors are often expressed in the same cells of many different tissues, including breast tumors, and since different dimer pairs have distinct genomic targets . Of particular interest is the observation that genistein, a compound originally thought to contribute to decreased breast cancer risk, specifically induces ERα homodimerization and transcriptional activity . This observation correlates well with more recent studies demonstrating that genistein is not effective in the prevention of breast cancer [59, 60]. Conversely, liquiritigenin is a highly selective ERβ agonist which does not stimulate ERα positive tumor formation  or ERα homodimerization  suggesting that it may serve as a suppressor of proliferation in ERβ expressing cells. The summation of these studies indicates that the identification of compounds which can specifically induce and/or activate ERα/β heterodimers or ERβ homodimers may have therapeutic potential. Endoxifen may serve as such a compound since it stabilizes ERβ protein levels and induces heterodimer formation in cells expressing both ER isoforms while simultaneously degrading ERα protein in ERβ negative cells .
Impact of ERβ positivity and increased endoxifen effectiveness
The identification of increased endoxifen effectiveness as an anti-estrogenic agent in the setting of ERβ is of significant clinical importance due to the fact that ERβ expression is reported to exist in approximately 75% of invasive breast cancers [33, 36, 37, 42, 62–64] and in a subset of tumors which are ERα negative [41, 45, 65]. The majority of reports suggest that the presence of ERβ in breast tumors correlates with improved rates of recurrence, disease-free survival and overall survival [29–38]; however, others indicate little correlation [39–41]. A few recent clinical studies have revealed that ERβ increases the effectiveness of tamoxifen therapy for breast cancer [44–46]. Given that endoxifen is being developed for the treatment of ER positive breast cancer, future studies should evaluate the association between ERβ expression and the activity of endoxifen in human breast tumors.
The present data indicate that ERβ enhances the anti-estrogenic actions of endoxifen in breast cancer cells. These data correlate well with the clinical studies demonstrating increased benefit from tamoxifen therapy in those patients whose tumors are ERβ positive and suggest that this benefit may be through the actions of endoxifen. The ability of low endoxifen concentrations to significantly inhibit estrogen induced gene expression and proliferation in ERβ expressing breast cancer cells also suggests that benefits from tamoxifen therapy may still be observed in patients characterized as poor metabolizers based on CYP2D6 genotype if their tumors are ERβ positive. Finally, these studies highlight the need to further investigate the role of ERβ in breast cancer, both as a prognostic and predictive factor, and lend additional support to the development of endoxifen as a novel therapeutic for the treatment of endocrine sensitive breast tumors.
cytochrome P450 2D6
Dulbecco's Modified Eagle's Medium/F12
estrogen response element
fetal bovine serum
National Cancer Institute
propyl pyrazole triol
real-time polymerase chain reaction
sodium dodecyl sulfate polyacrylamide gel electrophoresis
selective estrogen receptor modulator.
The authors would like to thank Elizabeth Bruinsma, Kenneth Peters, Kevin Pitel and Muzaffer Cicek for their excellent technical support and Jacquelyn House for her outstanding secretarial assistance. Additionally, we would like to thank the Mayo Clinic Advanced Genomic Technology Center Microarray Shared Resource for their invaluable contribution to this study. This work was supported by Susan G. Komen for the Cure KG100142 (JRH), a Breast Cancer Research Foundation grant (JNI and TCS), a generous gift from Bruce and Martha Atwater (TCS, JRH, MPG, JNI), a Breast Cancer SPORE (P50CA116201) Career Development Award (JRH) and the Mayo Foundation.
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