Y-box binding protein-1 serine 102 is a downstream target of p90 ribosomal S6 kinase in basal-like breast cancer cells
© Stratford et al.; licensee BioMed Central Ltd. 2008
Received: 26 May 2008
Accepted: 27 November 2008
Published: 27 November 2008
Basal-like breast cancers (BLBC) frequently overexpress the epidermal growth factor receptor (EGFR) and subsequently have high levels of signaling through the MAP kinase pathway, which is thought to contribute to their aggressive behavior. While we have previously reported the expression of Y-box binding protein-1 (YB-1) in 73% of BLBC, it is unclear whether it can be regulated by a component of the MAP kinase signaling pathway. Phosphorylation of YB-1 at the serine 102 residue is required for transcriptional activation of growth-enhancing genes, such as EGFR. Using Motifscan we identified p90 ribosomal S6 kinase (RSK) as a potential candidate for activating YB-1.
Inhibition of RSK1 and RSK2 was achieved using siRNA and the small molecule SL0101. RSK1, RSK2, activated RSK and kinase-dead RSK were expressed in HCC1937 cells. Kinase assays were performed to illustrate direct phosphorylation of YB-1 by RSK. The impact of inhibiting RSK on YB-1 function was measured by luciferase assays and chromatin immunoprecipitation.
Using an in vitro kinase assay, RSK1 and RSK2 were shown to directly phosphorylate YB-1. Interestingly, they were more effective activators of YB-1 than AKT or another novel YB-1 kinase, PKCα. Phosphorylation of YB-1 (serine 102 residue) is blocked by inhibition of the MAP kinase pathway or by perturbing RSK1/RSK2 with siRNA or SL0101. In immortalized breast epithelial cells where RSK is active yet AKT is not, YB-1 is phosphorylated. Supporting this observation, RSK2-/- mouse embryo fibroblasts lose the ability to phosphorylate YB-1 in response to epidermal growth factor. This subsequently interfered with the ability of YB-1 to regulate the expression of EGFR. The RSK inhibitor SL0101 decreased the ability of YB-1 to bind the promoter, transactivate and ultimately reduce EGFR expression. In concordance with these results the expression of constitutively active RSK1 increased YB-1 phosphorylation, yet the kinase-dead RSK did not.
We therefore conclude that RSK1/RSK2 are novel activators of YB-1, able to phosphorylate the serine 102 residue. This provides a newly described mechanism whereby YB-1 is activated in breast cancer. This implicates the EGFR/RSK/YB-1 pathway as an important component of BLBC, providing an important opportunity for therapeutic intervention.
Basal-like breast cancers (BLBC) are clinically challenging cases that are not amenable to current targeted therapies due to the absence of estrogen receptor or HER-2 expression. Treatment therefore depends on aggressive chemotherapy, yet relapse rates and overall survival are poor. Identification of potential therapeutic targets is an ongoing challenge.
Y-box binding protein-1 (YB-1) is an oncogenic transcription/translation factor that is overexpressed in a number of cancer types, including breast cancer [1, 2], prostate cancer , bone cancer , lung cancer [5, 6], colon cancer , muscle cancer  and, most recently, pediatric brain tumours . In particular, we have shown YB-1 to be expressed in a high proportion of BLBC , where it is associated with high rates of relapse . Overexpression of YB-1 in breast cancer cells results in an increase in monolayer and enhanced anchorage independent growth . Further, a study by Bergmann and colleagues demonstrated that targeted expression of YB-1 in the mammary gland of mice resulted in tumour formation with 100% penetrance . Conversely, we find that suppressing YB-1 using RNA interference inhibits tumour cell growth in vitro  and in vivo . The role of YB-1 in promoting growth of breast cancer cells stems from its original identification as a DNA binding protein, interacting with the regulatory elements of epidermal growth factor receptor (EGFR), HER-2  and c-MYC .
In the succeeding 20 years since these findings, many more growth-promoting genes have been identified as YB-1 targets, including topoisomerase II , DNA polymerase alpha and proliferating cell nuclear antigen (PCNA)  to name just a few examples. The question that arises is how YB-1 becomes activated to induce the expression of these genes so central to the development of cancer.
We previously demonstrated the importance of phosphorylation at the serine 102 residue (S102) to the functions of YB-1 [1, 2]. This site lies in the highly conserved cold-shock domain and is key for YB-1 nuclear localization and its ability to transform cells . Recent studies have provided evidence for the vital role of phosphorylation this residue plays in the binding of YB-1 to, and the regulation of, the EGFR promoter and subsequent protein production [1, 2]. In short, we have shown MCF-7 breast cancer cells overexpressing YB-1 have elevated levels of EGFR mRNA and protein . Subsequently we reported that YB-1 bound the EGFR promoter in BLBC cells in a S102 phosphorylation-dependent manner . Several studies have also implicated the importance of S102 phosphorylation in promoting translation [17, 18]. Phosphorylation of S102 is therefore important for activating the transcriptional and translational control imparted by YB-1.
We previously demonstrated that AKT binds directly to YB-1 and phosphorylates the S102 site , an observation subsequently confirmed in NIH3T3 cells . A recent study by Basaki and colleagues showed that serum stimulated YB-1 nuclear localization in ovarian cells and, further, this translocation was prevented by inhibiting AKT .
The Phosphatidylinositol-3 kinase (PI3K) pathway may not be the major contributor to growth in BLBC. EGFR is expressed in at least 50% of BLBC  and was recently used as one of five markers to identify aggressive BLBC . We previously found that, by inhibiting EGFR with Iressa, we could slow the growth of BLBC cells . Since this receptor signals through the MAP kinase pathway, we questioned whether other kinases are able to phosphorylate this key residue. We therefore took a bioinformatics approach to identify potential candidates, and determined that p90 ribosomal S6 kinase (RSK) may also phosphorylate YB-1 at S102 . RSK1 to RSK4 are members of the AGC serine/threonine superfamily of kinases  that lie downstream of the MAP kinase pathway. RSKs are a direct substrate of ERK , but also require phosphorylation by phosphoinositide-dependent protein kinase-1 (PDK-1)  and subsequent autophosphorylation steps .
The importance of RSK family members in diseases such as cancer is just being appreciated. Of the four isoforms, RSK1 and RSK2 are the most well characterized, and overexpression has been associated with multiple cancer types such as prostate cancer  and those of hematologic malignancies . Recent studies showed that RSK3 may actually be a tumour suppressor in ovarian cancer , and RSK4 differed from the other isoforms in that it was expressed at low levels and was constitutively active . In breast cancer, a small study carried out by Smith and colleagues found that both RSK1 and RSK2 expression levels were elevated in ~50% of tumours compared with control cases (n = 12 controls, n = 48 cancers) . We questioned whether RSK1 or RSK2 may play a role in BLBC because they lie in the MAP kinase pathway, which is commonly activated in this type of breast cancer due to overexpression of EGFR. In light of studies showing that RSK phosphorylates other transcription factors such as creb, c-fos  and the estrogen receptor , we contended that it may play an important role in regulating YB-1.
Materials and methods
Cell lines and reagents
The SUM149, HCC1937, MDA-MB-231 and MDA-MB-468 cells were used as models of BLBC; all are estrogen receptor negative, progesterone receptor negative and HER-2 negative . SUM149 cells were purchased from Asterand (Ann Arbor, MI, USA) and were cultured as previously described . MDA-MB-231 and MDA-MB-468 (both American Type Culture Collection, Manassas, VA, USA) cells were grown in DMEM (Gibco/Invitrogen, Burlington, ON, Canada) supplemented with 10% FBS and 100 units/ml penicillin/streptomycin. HCC1937 cells (kind donation from WD Foulkes, McGill University, QC, Canada) were cultured in RPMI-1640 media supplemented with 5% FBS, 10 mM HEPES, 4.5 g/l glucose (Sigma, Oakville, ON, Canada), 1 mM sodium pyruvate (Sigma) and 100 units/ml penicillin/streptomycin.
HTR-YB#5 (HTRY) are human mammary epithelial cells immortalized with HPV16, and express YB-1 if induced with tetracycline . These were maintained in the same media as SUM149 cells supplemented with 10 ng/ml epidermal growth factor (provided by author IMB). RSK1/RSK2 specific inhibitor SL0101 (Toronto Research Chemicals Inc., North York, ON, Canada) was dissolved in methanol [31, 36, 37], and PD098059 (Cell Signaling Technologies, Danvers, MA, USA), phorbal 12-myristate 13-acetate (PMA) (Sigma) and epidermal growth factor (EGF) were dissolved in dimethylsulfoxide (DMSO).
Growth factor stimulation and drug treatments
SUM149 cells were seeded at a density of 4 × 105 cells in a six-well plate. Cells were subsequently serum-starved for 24 hours prior to 6 hours treatment with vehicle, PD098059 (20 μM) or SL0101 (50 μM). Treated cells were stimulated with the following growth factors for 15 minutes before harvesting; 5% FBS/Ham's/F12 (serum stimulation), EGF (25 ng/ml) and PMA (50 ng/ml), lysed in egg lysis buffer (ELB) and subjected to western blot analysis . In all other experiments, HCC1937, MDA-MB-231 and HTRY cells were treated with 100 μM SL0101 and the SUM149 cells with 50 μM for 6 hours. The experiment was performed three times.
Protein extraction and western blot analysis
Protein was extracted from log-growing cells in ELB , supplemented with protease and phosphatase inhibitors, and quantified using the Bradford assay (Biorad, Hercules, CA, USA). Immunoblotting was performed as previously described . Specific proteins were detected using the following antibodies: EGFR, 1:1,000 (Stressgen, San Diego, CA, USA); ERK, 1:1,000 (p44/42 MAP kinase; Cell Signaling Technology, Danvers, MA, USA); RSK1, 1:1,000 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); RSK2, 1:500 (Santa Cruz Biotechnology); YB-1, 1:2,000 (Abcam, Cambridge, MA, USA); P-ERK, 1:500 (Cell Signaling Technology); P-RSKS380, 1:1,000 (Cell Signaling Technology); P-YB-1S102, 1:1,500 (Cell Signaling Technology, Danvers, MA, USA); Vinculin, 1:1,000 (Upstate, Temecula, CA, USA); and Pan-actin, 1:1,000 (Cell Signaling Technology). Densitometry was performed where appropriate.
RSK/AKT kinase assay
A synthetic peptidomimetic of the YB-1 S102 region was manufactured by Sigma with the sequence PRKYLRSVG-COOH. Kinase assays for RSK1, RSK2, AKT1 and PKCα were carried out on the peptide and activity was compared with an optimized control target (100% activity) (SignalChem, Richmond, BC, Canada). Control target sequences were as follows: RSK, KRRRLASLR; AKT1, CKRPRAASFAE; PKC, KRREILSRRPSYR.
The kinase assay reactions consisted of active protein kinase (250 ng/assay), substrate (optimized, YB-1 peptide or assay buffer) (40 μM), radiolabeled 33P-ATP (50 μM in kinase assay buffer; 25 mM MOPS, 12.5 mM β-glycerol phosphate, 25 mM MgCl2, 5 mM ethylene glycol tetraacetic acid (EGTA), 2 mM ethylenediamine tetraacetic acid, 0.25 mM dithiothreitol) to a final volume of 25 μl. Assays were performed at 30°C for 60 minutes, and then the reaction mixture was dotted on phosphocellulose P81 paper and the radioactivity measured. Activity greater than 5% of the optimized positive control is considered highly significant.
RSK1/YB-1 kinase assay from cell lysates
MCF-7 cells stably expressing Flag-YB-1 were serum starved for 16 hours prior to being lysed in radio immunoprecipitation assay (RIPA) buffer. As described above, 500 μg lysate was precleared with protein G agarose for 2 hours. YB-1 was then immunoprecipitated from the cells by overnight incubation at 4°C with 5 μg anti-Flag M2 antibody (Sigma) followed by 2 hours of incubation with protein G agarose. Complexes were then collected by centrifugation and washed firstly in Tris-buffered saline/1% NP40 and then once in modified wash buffer (100 mM Tris, pH 7.4, 50 mM NaCl, 1.5 mM MgCl2, 1 mM ethylenediamine tetraacetic acid, 0.5% NP40). YB-1 was isolated from protein G through incubation in 0.1 M glycine, pH 3.5, for 5 min at room temperature. Kinase assays were performed for RSK1 as described above.
Log-growing SUM149 cells were lysed in RIPA buffer supplemented with protease inhibitors. Cell lysates were subjected to a Bradford assay for quantification and 500 μg protein was used in subsequent immunoprecipitations. For YB-1 pull-down, lysates were precleared with 60 μl PrecipHen beads (previously described ) for 2 hours at 4°C with rotation, and the supernatants then incubated with IgY or chicken anti-YB-1 antibodies (5 μg) overnight at 4°C with rotation. Immunocomplexes were collected on PrecipHen beads after incubation at 4°C for 3 hours, by centrifugation. The beads were washed once with PBS/1% NP40, twice with wash buffer (100 mM Tris, pH 7.4, 100 mM NaCl, 1.5 mM MgCl2, 1 mM ethylenediamine tetraacetic acid, 0.5% NP40) and the proteins eluted by boiling in 5× loading dye for 5 minutes.
Similarly, for total RSK1/RSK2 immunoprecipitations, lysates (500 μg) were precleared with 35 μl protein G agarose for 2 hours prior to incubation with either control IgG or RSK1 or RSK2 antibodies (5 μg) (Santa Cruz Biotechnology) for 16 hours at 4°C with rotation. Immunocomplexes were retrieved through the addition of protein G agarose for 2 hours. Immunoprecipitated proteins were resolved on acrylamide gels and immunoblotted as described above. Horseradish peroxidase protein A was used as the secondary antibody to avoid detection of denatured immunoglobulins (1:2,000; Amersham Biosciences, Piscataway, NJ, USA).
RSK1 and RSK2 siRNA transfection
SUM149 cells (4 × 105/well) were transfected with 20 nM siRNA (Qiagen, Mississauga, ON, Canada) using Hiperfect (Qiagen). The fast-forward protocol was followed as described by the manufacturer. RSK1 and RSK2 siRNA sequences were as previously described .
HCC1937 cells were seeded at a density of 4 × 105 in a six-well plate 24 hours prior to transfection. Cells were transfected with 2 μg plasmid DNA using 10 μl Lipofectamine 2000/well as per instructions, and were lysed at 24 hours. Plasmid constructs for RSK overexpression studies were empty vectors (pRK7 and pKH3), pKH3-avRSK1, pKH3-mRSK2, pRK7-Myr-avRSK1 and pKH3-avRSK1(K112/464R) (kinase-dead) as previously described . The experiment was carried out three times.
RSK2-/-mouse embryo fibroblasts
Wild-type and RSK2-/- mouse embryo fibroblasts (MEFs) were cultured as described previously , and were stimulated with EGF (10 ng/ml) and cell lysates collected at 5, 15, 30, 60 and 120 minutes (kind donation from Dr YY Cho, University of Minnesota, Austin, MN, USA). Two sets of samples were analyzed.
SUM149 cells were plated in six-well plates (4 × 105 cells/well) and transfected with a luciferase construct containing the first 1 kb of the EGFR promoter (pER1) (kind gift from Alfred C. Johnson US National Cancer Institute, Bethesda, MD, USA – previously described in [1, 41]). Cells were transfected with a total of 1.5 μg DNA using Lipofectamine 2000 (Invitrogen). To account for transfection efficiency, cells were co-transfected with a renilla-expressing plasmid (pRL-TK, 10:1 luciferase:renilla; Promega). After 18 hours, cells were treated with vehicle or SL0101 (50 μM) for 6 hours prior to harvesting in 1 × passive lysis buffer (Promega). Luciferase activity was measured and normalized to the renilla reading from the same sample.
SUM149 cells (1 × 107 cells) were treated with vehicle, PD098059 (20 μM) or SL0101 (50 μM) for 6 hours. Crosslinks were established between protein and DNA following 15 minutes of incubation with 1% formaldehyde. Cells were washed and collected by centrifugation. Chromatin immunoprecipitation with anti-P-YB-1 antibody (gift from Dr P Mertens, University Hospital RWTH – Aachen, Aachen, Germany) was carried out as described previously [1, 2]. The resulting DNA was amplified using the EGFR2a primers (previously described [1, 2]).
YB-1 is phosphorylated by the MAP kinase pathway
RSK phosphorylates YB-1 at serine residue 102
Activity of RSK1, AKT1 and PKCα against the Y-box binding factor-1(YB-1) serine 102 residue peptide compared with the optimized positive control substrate
Activity against YB-1 peptide compared with control (%)
80 ± 1.04
78 ± 0.78
7 ± 0.7
19 ± 1.14
Inhibition of RSK functionally inactivates YB-1
Inhibition of RSK2 by siRNA in SUM149 cells (Figure 4c) led to a decrease in EGFR expression. This downregulation was mirrored in HTRY and MDA-MB-231 cells following treatment with SL0101 (Figure 4d); densitometric analysis for MDA-MB-231 gave a 35% decrease. We thereby conclude that there is a feed-forward signaling pathway in BLBC where EGF binds to the EGFR, which in turn leads to activation of the MAP kinase/RSK pathway resulting in phosphorylation of YB-1 at S102. Activated AKT and PKCα also have the ability to activate YB-1. Following this, P-YB-1S102 binds to and transactivates the EGFR gene, further fueling the growth potential of BLBC (Figure 4e).
We reveal for the first time that phosphorylation of YB-1 at the S102 location is not only carried out by the PI3K cascade but that signaling through the MAP kinase pathway can also activate this transcription factor. This is particularly relevant in BLBC, where EGFR is overexpressed in over one-half of the cases. More specifically it is the serine/threonine kinases RSK1 and RSK2 that are able to phosphorylate YB-1 at the key S102 residue in BLBC cells. Not only do we identify RSK1 and RSK2 as proteins that can directly interact and phosphorylate YB-1, but they have a much greater efficiency towards the target than AKT1 does. In fact, we also identified PKCα as having greater kinase activity towards YB-1 than AKT1, a finding that warrants future investigation. Phosphorylated RSK is also expressed in cell lines where we find abundant P-YB-1S102 and a lack of active AKT; in particular the MDA-MB-231 cells and the immortalized human mammary epithelial cells, where we were unable to detect any P-AKTS473. The RSK1/RSK2-specific inhibitor SL0101 [31, 43], as well as RSK1-targeted or RSK2-targeted siRNA, were able to reduce the phosphorylation of YB-1 at S102 even following induction by the classic tumour promoter PMA. Furthermore, we observed a reduced level of P-YB-1S102 in RSK2-/- MEFs. Finally, inhibition of RSK prevented P-YB-1S102 binding to the EGFR promoter and ultimately reduced the protein expression of this receptor tyrosine kinase.
Our data are consistent with a recent study by Hoadley and colleagues reporting that EGFR and genes encoding components of the MAP kinase pathway were associated with the basal-like subtype, while AKT1 was not . Interestingly, we found in our four BLBC cell lines that ERK2 expression was predominantly expressed over ERK1. This is in concordance with the analysis observed by Hoadley and colleagues, which shows expression of ERK2 was increased in the BLBC cluster, but this was not the case for ERK1 . It is thus conceivable that ERK2 may activate RSK and therefore YB-1 in basal-like tumours. In this context it is also of interest that we in fact find YB-1S102 to be a better substrate for RSK1 and RSK2 than AKT1. ERK2 may also directly phosphorylate YB-1 and therefore promotes its ability to transactivate target genes. In support of this idea, ERK2 promotes the transactivation of vascular endothelial growth factor by YB-1 . This occurs when ERK2 phosphorylates the N-terminal region of YB-1; the region of the protein required for gene transactivation . More recently, we identified a putative ERK phosphorylation site at serine 36 in this same region of the protein using Motif Scanner ; however, this has not been validated experimentally. While speculative at this point, if ERK does phosphorylate the transactivating domain of YB-1 this could explain why inhibiting ERK activity with PD098059 was better than SL0101 at suppressing EGFR reporter activity. In theory, inhibiting ERK2 would directly decrease phosphorylation of YB-1 at S36 at the N-terminal and indirectly block RSK from phosphorylating S102. These studies indicate that the MAP kinase pathway would have broad effects on YB-1.
While the emphasis of this study has been on BLBC, EGFR is equally important in promoting growth signals in other types of breast cancer. For example, EGFR forms heterodimers with Her-2 to engage signaling through either the MAPK or AKT pathways, which perhaps also involves RSK as well as AKT. This obviously could be important in stimulating the growth of breast cancer cells harboring amplified Her-2. Beyond breast cancer, we suspect that the relationship between RSK and YB-1 could be important in other malignancies. A study by Cho and colleagues demonstrated that RSK2 was a transforming gene, since stable expression in skin cells increased the colony number in anchorage-independent conditions . Conversely, knockdown of RSK2 reduced colony formation even in the presence of constitutively active oncogenic Ras . Other studies implicate RSK2 in transmitting the prosurvival and proliferative signals from oncogenic mutant receptor tyrosine kinase FGFR3 in multiple myeloma, resulting in cell transformation [28, 46]. Interestingly, YB-1 has been implicated in the survival and progression of multiple myeloma cells – the expression correlating with rapid proliferation and poor differentiation . We therefore postulate a model where RSK is activated through aberrant tyrosine kinase signaling, resulting in the subsequent phosphorylation of YB-1. In this way the cell will be influenced by any number of a diverse collection of genes that YB-1 has been shown to regulate, such as EGFR [1, 14], Her-2 , topoisomerase II [5, 7] and the multidrug resistance gene [48, 49]. This regulation in fact may result in a positive feedback loop in the case of genes such as EGFR.
Beyond regulating transcription, YB-1 also promotes translation, alternative splicing, RNA transport and DNA repair [17, 18, 50–52]. Whether phosphorylation of YB-1 at S102 is important for these events is not known. Interestingly, RSK itself promotes translation through several mechanisms [23, 39, 53, 54]; therefore, the role of these two proteins acting together in this process needs to be further investigated.
We conclude that RSK1 and RSK2 are able to phosphorylate YB-1S102, providing a newly described mechanism whereby this transcription factor is activated in breast cancer. In fact, RSK activates YB-1 more effectively than AKT and may therefore be the major facilitator of YB-1 function in BLBC. Interest in developing small molecules against RSK has increased over the past 2 years, and we believe this could be an important opportunity for therapeutic intervention. As RSK has never before been associated with BLBC, we therefore introduce a new mechanistic understanding and potentially a therapeutic strategy for treating this aggressive disease.
basal-like breast cancers
Dulbecco's modified eagle medium
epidermal growth factor
epidermal growth factor receptor
egg lysis buffer
extracellular signal-regulated kinases
fetal bovine serum
mouse embryo fibroblast
protein kinase C alpha
phorbal 12-myristate 13-acetate
radio immunoprecipitation assay
p90 ribosomal S6 kinase
serine 102 residue
small interfering RNA
Y-box binding factor-1.
The authors are very grateful to Dr J Sanghera and Mr R Li at SignalChem (Richmond, BC, Canada) for carrying out the RSK1, AKT1 and PKCα kinase assays on the YB-1 peptide. Research in the laboratory of SED is supported by National Cancer Institute of Canada (NCIC), the Canadian Breast Cancer Research Alliance and postdoctoral fellowships from the Canadian Breast Cancer Foundation and the Child and Family Research Institute. Research in the laboratory of PPR is supported by a Terry Fox Foundation grant obtained through the NCIC and a Career Development Award from the Human Frontier Science Program Organization. PPR holds a Canada Research Chair in Signal Transduction and Proteomics. IMB is supported by an R01.
- Stratford AL, Habibi G, Astanehe A, Jiang H, Hu K, Park E, Shadeo A, Buys TPH, Lam W, Pugh T, Marra MA, Nielsen TO, Klinge U, Mertens PR, Aparicio S, Dunn SE: Epidermal growth factor receptor (EGFR) is transcriptionally induced by the Y-box binding protein-1 (YB-1) and can be inhibited with Iressa in basal-like breast cancer providing a potential target for therapy. Breast Cancer Res. 2007, 9: R61-10.1186/bcr1767.View ArticlePubMedPubMed CentralGoogle Scholar
- Wu J, Lee C, Yokom D, Jiang H, Cheang MCU, Yorida E, Turbin D, Berquin IM, Mertens PR, Iftner T, Gilks B, Dunn SE: Disruption of the Y-box binding protein-1 (YB-1) results in suppression of the epidermal growth factor receptor and Her-2. Cancer Res. 2006, 66: 4872-4879. 10.1158/0008-5472.CAN-05-3561.View ArticlePubMedGoogle Scholar
- Gimenez-Bonafe P, Fedoruk MN, Whitmore TG, Akbari M, Ralph JL, Gleave ME, Nelson CC: YB-1 is upregulated during prostate cancer tumor progression and increases P-glycoprotein activity. Prostate. 2004, 59: 337-349. 10.1002/pros.20023.View ArticlePubMedGoogle Scholar
- Oda Y, Sakamoto A, Shinohara N, Ohga T, Uchiumi T, Kohno K, Tsuneyoshi M, Kuwano M, Iwamoto Y: Nuclear expression of YB-1 protein correlates with P-glycoprotein expression in human osteocarcinoma. Clin Cancer Res. 1998, 4: 2273-2277.PubMedGoogle Scholar
- Gu C, Oyama T, Osaki T, Kohno K, Yasumoto K: Expression of Y box-binding protein 1 correlates with DNA topoisomerase II alpha and proliferating cell nuclear antigen expression in lung cancer. Anticancer Res. 2001, 21: 2357-2362.PubMedGoogle Scholar
- Shibahara K, Sugio K, Osaki T, Uchiumi T, Maehara Y, Kohno K, Yasumoto K, Sugimachi K, Kuwano M: Nuclear expression of the Y-box binding protein, YB-1, as a novel marker of disease progression in non-small cell lung cancer. Clin Cancer Res. 2001, 7: 3151-3155.PubMedGoogle Scholar
- Shibao K, Takano H, Nakayama Y, Okazaki K, Nagat N, Izumi H, Uchiumi T, Kuwano M, Kohno K, Itoh H: Enhanced coexpression of YB-1 and DNA topoisomerase II a genes in human colorectal carcinomas. Int J Cancer. 1999, 83: 732-737. 10.1002/(SICI)1097-0215(19991210)83:6<732::AID-IJC6>3.0.CO;2-#.View ArticlePubMedGoogle Scholar
- Oda Y, Ohishi Y, Saito T, Hinoshita E, Uchiumi T, Kinukawa N, Iwamoto Y, Kohno K, Kuwano M, Tsuneyoshi M: Nuclear expression of Y-box binding protein-1 correlates with P-glycoprotein and topoisomerase II alpha expression and with poor prognosis in synovial sarcoma. J Pathol. 2003, 199: 251-258. 10.1002/path.1282.View ArticlePubMedGoogle Scholar
- Faury D, Nantel A, Dunn SE, Guiot M-C, Haque T, Hauser P, Garami M, Bognár L, Hanzély Z, Liberski PP, Lopez-Aguilar E, Valera ET, Tone LG, Carret A-S, Del Maestro RF, Gleave M, Montes J-L, Pietsch T, Albrecht S, Jabado N: Molecular profiling identifies prognostic subgroups of pediatric glioblastoma and shows increased YB-1 expression in tumors. J Clin Oncol. 2007, 25: 1196-1208. 10.1200/JCO.2006.07.8626.View ArticlePubMedGoogle Scholar
- Habibi G, Leung S, Law JH, Gelmon K, Masoudi H, Turbin D, Pollak M, Nielsen TO, Huntsman D, Dunn SE: Re-defining prognostic factors for breast cancer: YB-1 is a stronger predictor of relapse and disease specific survival than estrogen receptor or HER-2 across all tumor subtypes. Breast Cancer Res. 2008, 10: R86-10.1186/bcr2156.View ArticlePubMedPubMed CentralGoogle Scholar
- Sutherland BW, Kucab JE, Wu J, Lee C, Cheang MCU, Yorida E, Turbin D, Dedhar S, Nelson CC, Pollack M, Grimes HL, Miller K, Badve S, Huntsman D, Gilks B, Chen M, Pallen CJ, Dunn SE: Akt phosphorylates the Y-box binding protein 1 at Ser102 located in the cold shock domain and affects the anchorage-independent growth of breast cancer cells. Oncogene. 2005, 24: 4281-4292. 10.1038/sj.onc.1208590.View ArticlePubMedGoogle Scholar
- Bergmann S, Royer-Pokora B, Fietze E, Jurchott K, Hildebrandt B, Trost D, Leenders F, Claude J-C, Theuring F, Bargou R, Dietel M, Royer H-D: YB-1 provokes breast cancer through the induction of chromosomal instability that emerges from mitotic failure and centrosome amplification. Cancer Res. 2005, 65: 4078-4087. 10.1158/0008-5472.CAN-04-4056.View ArticlePubMedGoogle Scholar
- Lee C, Dhillon J, Wang MYC, Gao Y, Hu K, Park E, Astanehe A, Hung M-C, Eirew P, Eaves CJ, Dunn SE: Targeting YB-1 in HER-2 over-expressing breast cancer cells induces apoptosis via the mTOR/STAT3 pathway and suppresses tumor growth in mice. Cancer Res. 2008, 68: 8661-8666. 10.1158/0008-5472.CAN-08-1082.View ArticlePubMedGoogle Scholar
- Sakura H, Maekawa T, Imamoto F, Yasuda K, Ishii S: Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology. Gene. 1988, 73: 499-507. 10.1016/0378-1119(88)90514-8.View ArticlePubMedGoogle Scholar
- Kolluri R, Kinniburgh AJ: Full length cDNA sequence encoding a nuclease-sensitive element DNA binding protein. Nucleic Acid Res. 1991, 19: 4771-10.1093/nar/19.17.4771.View ArticlePubMedPubMed CentralGoogle Scholar
- Kohno K, Izumi H, Uchiumi T, Ashizuka M, Kuwano M: The pleiotropic functions of the Y-box-binding protein, YB-1. BioEssays. 2003, 25: 691-698. 10.1002/bies.10300.View ArticlePubMedGoogle Scholar
- Bader AG, Vogt PK: Phosphorylation by Akt disables the anti-oncogenic activity of YB-1. Oncogene. 2008, 27: 1179-1182. 10.1038/sj.onc.1210719.View ArticlePubMedGoogle Scholar
- Evdokimova V, Ruzanov P, Anglesio MS, Sorokin AV, Ovchinnikov LP, Buckley J, Triche TJ, Sonenberg N, Sorensen PHB: Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species. Mol Cell Biol. 2006, 26: 277-292. 10.1128/MCB.26.1.277-292.2006.View ArticlePubMedPubMed CentralGoogle Scholar
- Basaki Y, Hosoi F, Oda Y, Fotovati A, Maruyama Y, Oie S, Ono M, Izumi H, Kohno K, Sakai K, Shimoyama T, Nishio K, Kuwano M: Akt-dependent nuclear localization of Y-box-binding protein 1 in acquisition of malignant characteristics by human ovarian cancer cells. Oncogene. 2007, 26: 2736-2746. 10.1038/sj.onc.1210084.View ArticlePubMedGoogle Scholar
- Nielsen TO, Cheang MC, Hsu FD, Turbin D, Hu XJ, Norris BD, Speers CH, Olivotto IA, Perou CM: Epidermal growth factor receptor and basal breast cancer: prognosis on a large population based series. Clin Cancer Res. 2004, 10: 5367-5374. 10.1158/1078-0432.CCR-04-0220.View ArticlePubMedGoogle Scholar
- Cheang MCU, Voduc D, Bajdik C, Leung S, McKinney S, Chia SK, Perou CM, Nielsen TO: Basal-like breast cancer defined by five biomarkers has superior prognostic value than triple-negative phenotype. Clin Cancer Res. 2008, 14: 1368-1376. 10.1158/1078-0432.CCR-07-1658.View ArticlePubMedGoogle Scholar
- Wu J, Stratford AL, Astanehe A, Dunn SE: YB-1 is a transcription/translation factor that orchestrates the oncogenome by hardwiring signal transduction to gene expression. Transl Oncogenomics. 2007, 2: 1-17.Google Scholar
- Carriere A, Ray H, Blenis J, Roux PP: The RSK factors of activating the Ras/MAPK signaling cascade. Front Biosci. 2008, 13: 4258-4275. 10.2741/3003.View ArticlePubMedGoogle Scholar
- Dalby KN, Morrice N, Caudwell FB, Avruch J, Cohen P: Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK. J Biol Chem. 1998, 273: 1496-1505. 10.1074/jbc.273.3.1496.View ArticlePubMedGoogle Scholar
- Jensen CJ, Buch M-B, Krag TO, Hemmings BA, Gammeltoft S, Frodin M: 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1. J Biol Chem. 1999, 274: 27168-27176. 10.1074/jbc.274.38.27168.View ArticlePubMedGoogle Scholar
- Fisher TL, Blenis J: Evidence for two catalytically active kinase domains in pp90rsk. Mol Cell Biol. 1996, 16: 1212-1219.View ArticlePubMedPubMed CentralGoogle Scholar
- Clark DE, Errington TM, Smith JA, Frierson HF, Weber MJ, Lannigan DA: The serine/threonine protein kinase, p90 ribosomal S6 kinase, is an important regulator of prostate cancer cell proliferation. Cancer Res. 2005, 65: 3108-3116.PubMedGoogle Scholar
- Cuadrado A, Nebreda AR: New insights into RSK activation and hematopoietic cancer. Cancer Cell. 2007, 12: 187-189. 10.1016/j.ccr.2007.08.017.View ArticlePubMedGoogle Scholar
- Bignone PA, Lee KY, Liu Y, Emilion G, Finch J, Soosay AER, Charnock FML, Beck S, Dunham I, Mungall AJ, Ganesan TS: RPS6KA2, a putative tumour suppressor gene at 6q27 in sporadic epithelial ovarian cancer. Oncogene. 2007, 26: 683-700. 10.1038/sj.onc.1209827.View ArticlePubMedGoogle Scholar
- Dummler BA, Hauge C, Silber J, Yntema HG, Kruse LS, Kofoed B, Hemmings BA, Alessi DR, Frodin M: Functional characterization of human RSK4, a new 90-kDa ribosomal S6 kinase, reveals constitutive activation in most cell types. J Biol Chem. 2005, 280: 13304-13314. 10.1074/jbc.M408194200.View ArticlePubMedGoogle Scholar
- Smith JA, Poteet-Smith CE, Xu Y, Errington TM, Hecht SM, Lannigan DA: Identification of the first specific inhibitor of p90 ribosomal S6 kinase (RSK) reveals an unexpected role for RSK in cancer cell proliferation. Cancer Res. 2005, 65: 1027-1034. 10.1158/0008-5472.CAN-05-0169.View ArticlePubMedGoogle Scholar
- Chen R-H, Abate C, Blenis J: Phosphorylation of the c-Fos transrepression domain by mitogen-activated protein kinase and 90-kDa ribosomal S6 kinase. Proc Natl Acad Sci USA. 1993, 90: 10952-10956. 10.1073/pnas.90.23.10952.View ArticlePubMedPubMed CentralGoogle Scholar
- Joel PB, Smith J, Sturgill TW, Fisher TL, Blenis J, Lannigan DA: pp90rsk1 regulates estrogen receptor-mediated transcription through phosphorylation of Ser-167. Mol Cell Biol. 1998, 18: 1978-1984.View ArticlePubMedPubMed CentralGoogle Scholar
- Neve RM, Chin K, Fridlyand J, Yeh J, Baehner FL, Fevr T, Clark L, Bayani N, Coppe J-P, Tong F, Speed T, Spellman PT, DeVries S, Lapuk A, Wang NJ, Kuo W-L, Stilwell JL, Pinkel D, Albertson DG, Waldman FM, McCormick F, Dickson RB, Johnson MD, Lippman M, Ethier SP, Gazdar AF, Gray JW: A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. Cancer Cell. 2006, 10: 515-527. 10.1016/j.ccr.2006.10.008.View ArticlePubMedPubMed CentralGoogle Scholar
- Berquin IM, Pang B, Dzuibinski ML, Scott LM, Chen YQ, Nolan GP, Ethier SP: Y-box-binding protein 1 confers EGF independence to human mammary epithelial cells. Oncogene. 2005, 24: 3177-3186. 10.1038/sj.onc.1208504.View ArticlePubMedGoogle Scholar
- Maloney DJ, Hecht SM: Synthesis of a potent and selective inhibitor of p90 rsk. Org Lett. 2005, 7: 1097-1099. 10.1021/ol0500463.View ArticlePubMedGoogle Scholar
- Smith JA, Maloney DJ, Hecht SM, Lannigan DA: Structural basis for the activity of the RSK-specific inhibitor, SL0101. Bioorg Med Chem. 2007, 15: 5018-5034. 10.1016/j.bmc.2007.03.087.View ArticlePubMedGoogle Scholar
- Shahbazian D, Roux PP, Mieulet V, Cohen MS, Raught B, Taunton J, Hershey JW, Blenis J, Pende M, Sonenberg N: The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity. EMBO J. 2006, 25: 2781-2791. 10.1038/sj.emboj.7601166.View ArticlePubMedPubMed CentralGoogle Scholar
- Roux PP, Ballif BA, Anjum R, Gygi SP, Blenis J: Tumor-promoting phorbol esters and activated Ras inactivate the tuberous sclerosis tumor suppressor complex via p90 ribosomal S6 kinase. Proc Natl Acad Sci USA. 2004, 101: 13489-13494. 10.1073/pnas.0405659101.View ArticlePubMedPubMed CentralGoogle Scholar
- Cho Y-Y, Yao K, Kim H-G, Kang BS, Zheng D, Bode AM, Dong Z: Ribosomal S6 kinase 2 is a key regulator in tumor promoter-induced cell transformation. Cancer Res. 2007, 67: 8104-8112. 10.1158/0008-5472.CAN-06-4668.View ArticlePubMedPubMed CentralGoogle Scholar
- Nishi H, Nishi KH, Johnsen AC: Early growth response-1 gene mediates up-regulation of epidermal growth factor receptor expression during hypoxia. Cancer Res. 2002, 62: 827-834.PubMedGoogle Scholar
- Willmarth NE, Ethier SP: Autocrine and juxtacrine effects of amphiregulin on the proliferative, invasive, and migratory properties of normal and neoplastic human mammary epithelial cells. J Biol Chem. 2006, 281: 37728-37737. 10.1074/jbc.M606532200.View ArticlePubMedGoogle Scholar
- Bain J, Plater L, Elliott M, Shpiro N, Hastie J, McLauchlan H, Klevernic I, Arthur S, Alessi DR, Cohen P: The selectivity of protein kinase inhibitors: a further update. Biochem J. 2007, 408: 297-315. 10.1042/BJ20070797.View ArticlePubMedPubMed CentralGoogle Scholar
- Hoadley KA, Weigman VJ, Fan C, Sawyer LR, He X, Troester MA, Sartor CI, Rieger-House T, Bernard PS, Carey LA, Perou CM: EGFR associated expression profiles vary with breast tumor subtype. BMC Genomics. 2007, 8: 258-277. 10.1186/1471-2164-8-258.View ArticlePubMedPubMed CentralGoogle Scholar
- Coles LS, Bartley MA, Bert A, Hunter J, Polyak S, Diamond P, Vadas MA, Goodall GJ: A multi-protein complex containing cold chock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA: potential role in mRNA stabilization. Eur J Biochem. 2004, 271: 648-660. 10.1111/j.1432-1033.2003.03968.x.View ArticlePubMedGoogle Scholar
- Kang S, Dong S, Gu TL, Guo A, Cohen MS, Lonial S, Khoury HJ, Fabbro D, Gilliland DG, Bergsagel PL, Taunton J, Polakiewicz RD, Chen J: FGFR3 activates RSK2 to mediate hematopoietic transformation through tyrosine phosphorylation of RSK2 and activation of the MEK/ERK pathway. Cancer Cell. 2007, 12: 201-214. 10.1016/j.ccr.2007.08.003.View ArticlePubMedPubMed CentralGoogle Scholar
- Chatterjee M, Rancso C, Stuhmer T, Eckstein N, Andrulis M, Gerecke C, Lorentz H, Royer HD, Bargou RC: The Y-box binding protein YB-1 is associated with progressive disease and mediates survival and drug resistance in multiple myeloma. Blood. 2008, 111: 3714-3722. 10.1182/blood-2007-05-089151.View ArticlePubMedGoogle Scholar
- Goldsmith ME, Madden MJ, Morrow CS, Cowan KH: A Y-box consensus sequence is required for basal expression of the human multidrug resistance (mdr1) gene. J Biol Chem. 1993, 268: 5856-5860.PubMedGoogle Scholar
- Stein U, Jürchott K, Walther W, Bergmann S, Schlag PM, Royer HD: Hyperthermia-induced nuclear translation of transcription factor YB-1 leads to enhanced expression of multidrug resistance-related ABC transporters. J Biol Chem. 2001, 276: 28562-28569. 10.1074/jbc.M100311200.View ArticlePubMedGoogle Scholar
- Raffetseder U, Frye B, Rauen T, Jurchott K, Royer HD, Jansen PL, Mertens PR: Splicing factor SRp30c interaction with Y-box protein-1 confers nuclear Yb-1 shuttling and alternative splice site selection. J Biol Chem. 2003, 278: 18241-18248. 10.1074/jbc.M212518200.View ArticlePubMedGoogle Scholar
- Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA: The RNA bindng protein Yb-1 binds a/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4. EMBO J. 2001, 20: 3821-3830. 10.1093/emboj/20.14.3821.View ArticlePubMedPubMed CentralGoogle Scholar
- Graumann P, Marahiel MA: A case of convergent evolution of nucleic acid binding modules. Bioessays. 1996, 18: 309-315. 10.1002/bies.950180409.View ArticlePubMedGoogle Scholar
- Roux PP, Shahbazian D, Vu H, Holz MK, Cohen MS, Taunton J, Sonenberg N, Blenis J: RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation. J Biol Chem. 2007, 282: 14056-14064. 10.1074/jbc.M700906200.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang X, Li W, Williams M, Terada N, Alessi DR, Proud CG: Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase. EMBO J. 2001, 20: 4370-4379. 10.1093/emboj/20.16.4370.View ArticlePubMedPubMed CentralGoogle Scholar
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