Volume 2 Supplement 1

Second International Symposium on the Molecular Biology of Breast Cancer

Open Access

REAL Real-Time!

  • F Boeckman1,
  • L Tan1 and
  • K Hamby1
Breast Cancer Research20002(Suppl 1):P8.02

DOI: 10.1186/bcr174

Published: 12 March 2000

Full text

Recent advances in real-time PCR analysis have led to significant improvements in the accuracy and simplicity of DNA quantification and gene expression analysis. Here we demonstrate the new Bio-Rad iCycler iQ system capabilities. When PCR is conducted on 96 replicates, we achieve a uniformity with a CV of less than 1%, consistent with that of other well-known systems for real-time PCR analysis. We demonstrate the ability to distinguish a two-fold dilution series of human genomic DNA down to 125 genomic equivalents. We also show a wide dynamic range over which quantification is possible, beginning with plasmids or genomic DNA. The iCycler iQ system is designed to work with many detection strategies; here we show the iCycler iQs' ability to employ a variety of techniques, including SYBR Green I, TaqMan® and Molecular Beacons. Finally, the iCycler iQ's unique ability to analyse data at any point within a cycle or dwell time can be a significant advantage when evaluating certain detection chemistries including molecular beacons.

Authors’ Affiliations

(1)

Copyright

© Current Science Ltd 2000

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